Intended Use
Intended to test E.coli O157 in water, beverages, dairy products, meat, seafood, etc.
Principle
The E.coli O157 Rapid Test Kit is based on sandwich lateral flow immunochromatographic assay. The test cassette has a window for result reading, which has a T (test) line and a C (control) line. Both are invisible before testing. When the sample is applied into the sample well on the device, the liquid will laterally flow through the surface of the test strip and react with the pre-coated antibodies. If there is E.coli O157 present in the sample, the T line will become visible. On the contrary, when there is no E.coli O157 in the sample or its concentration is below the detection limit, the test line shows no color, indicating a negative result. While the C line should always appear in the test, which indicates a valid result. By this means, the device can indicate the presence of E.coli O157 in the sample.
Kit Contents, Storage and Shelf Life
The E.coli O157 Rapid Test Kit has the capacity for 10 determinations. The shelf life is 18 months when the kit is properly stored.
Kit Contents | Amount | Storage |
Test cassettes | 10 | 2-25˚C |
0157 Enrichment Broths | 25 mL x2 | |
Disposable pipettes | 10 | |
User manual | 1 |
Required Materials not Provided With the Kit
- Homogenizer● Sterilized normal saline ● Pipettes
- Vortex mixer ● Weighing scale