ćProduct Nameć
Generic Name: Monkeypox Virus Real-Time PCR Detection Kit
ćPacking Specificationsć
50Ā tests/kit
ćExpected Usageć
This kit is used for detection monkeypox virus DNA, and can be used for clinical auxiliary diagnosis of monkeypox virus.
ćPrinciple of Inspectionć
This kit uses polymerase chain reaction combined with Taqman technology to perform fluorescence PCR detection of specific DNA nucleic acid fragments of monkeypox virus.
ćMain Ingredientsć
Component |
50Ā tests/kit
|
|
Specification | Quantity | |
PCR Reaction Solution | 1014Ī¼L /Ā tube | 1Ā tube |
Enzyme Mix | 27Ī¼L /Ā tube | 1Ā tube |
Positive Control | 200Ī¼L /Ā tube | 1Ā tube |
Negative Control | 500Ī¼L /Ā tube | 1Ā tube |
ćStorage Conditions And Validity Periodć
The reagent is stored at -30Ā ~Ā -15āĀ and store itĀ in shadeĀ . The validity period is 12 months.
This product should be frozen and thawed no more than 5Ā times.
ćApplicable Instrumentć
Fluorescence quantitative PCR instrument: ABI 7500 fluorescence PCR instrument.; ABI Quantstudio5; Gentier 96R; SLAN-96P automatic medical PCR analysis system.
ćSample Requirementsć
- Applicable Sample
(1) Rash exudate: The rash exudate was swabbed with a sterile swab, placed in a sterile tube (containing 1ml of sterilized saline), and sent for examination under airtight conditions, and the specimen was immediately available for testing.
(2) Oropharyngeal swab: Wipe the swab slightly hard back and forward on both sides of the tonsil of the recipient at least 3 times, and then wipe it up and down the posterior pharyngeal wall at least 3 times, and dip the swab head into the tube containing virus preservation solution to tighten the tube lid.
- Sample Shipping and Storage
Strictly follow the relevant regulations in “specimen preservation” and “specimen submission for inspection” of clinical specimens.
Sample Storage: The sample to be tested can be subjected to nucleic acid extraction immediately,Ā if the sample needs to be tested within 24 hours, it can be stored at 2~8ā,if the sample cannot be detected within 24 hours, it can be stored at -20ā~ -80ā, try to avoid repeated freezing and thawing, resulting in false negatives.
Sample Shipping: Low temperature transportation is adopted, and the sample transportation should comply with the relevant national biosafety regulations on the second type of pathogenic microorganisms.
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