Intended Use

This kit is for the in vitro qualitative detection of Hantavirus (HV) IgG antibodies in human serum samples.Hantavirus is the pathogen that causes hemorrhagic fever with renal syndrome (HFRS), a global, zoonotic acute infectious disease characterized by fever, bleeding, and kidney damage. It has a rapid onset, rapid progression, and high mortality rate. my country accounts for over 90% of the world’s reported HFRS cases annually, making it one of the deadliest infectious diseases in my country. The main viruses causing HFRS include Hantavirus (HV), Seoulvirus (SEOV), Puumalavirus (PUUV), and Dobravavirus (DOBV), with HV and SEOV being the predominant types in my country.Hantaviruses are mainly distributed in Asia and the Balkans, and are transmitted to humans through direct contact with rodents or their excrement or secretions. Soldiers, farmers, and campers are particularly vulnerable.

Principle

This kit uses an indirect enzyme-linked immunosorbent assay (ELISA) to qualitatively detect Hantavirus IgG antibodies (HV-IgG) in human serum samples. The ELISA plate is coated with Hantavirus antigen. The Hantavirus antibodies in the test serum react with the coated antigen, and then bind to enzyme-labeled mouse anti-human IgG antibody, forming an antigen-antibody-enzyme-labeled antibody complex. The substrate TMB is added for color development, and the presence of Hantavirus IgG antibodies is determined by the OD value after colorimetric measurement on an ELISA reader.