The Typhoid IgG/IgM Rapid Test Kit is a rapid chromatographic immunoassay for the qualitative detection of antibodies (IgG and IgM) to Typhoid virus in Whole Blood /Serum / Plasma to aid in the diagnosis of Typhoid viral infection.
Typhoid fever is a bacterial disease, caused by Salmonella typhi. It is transmitted through the ingestion of food or drink contaminated by the faeces or urine of infected people. Symptoms usually develop 1–3 weeks after exposure, and may be mild or severe. They include high fever,malaise, headache, constipation or diarrhoea, rose-coloured spots on the chest, and enlarged spleen and liver. Healthy carrier state may follow acute illness. Typhoid IgG/IgM Rapid Test Kit is a simple, visual qualitative test that detects Typhoid antibodies in human Whole Blood/serum/plasma. The test is based on immunochromatography and can give a result within 15 minutes.
The Typhoid IgG/IgM Rapid Test Kit is a qualitative membrane strip based immunoassay for the detection of Typhoid antibodies (IgG and IgM) in Whole Blood /Serum / Plasma. The test device consists of: 1) a burgundy colored conjugate pad containing Typhoid recombinant envelope antigens conjugated with Colloid gold (Typhoid conjugates) and rabbit IgG-gold conjugates,2) a nitrocellulose membrane strip containing two test bands (T1 and T2 bands) and a control band (C band). The T1 band is pre-coated with the antibody for the detection of IgG anti-Typhoid, T2 band is coated with antibody for the detection of IgM anti-Typhoid, and the C band is pre-coated with goat anti rabbit IgG. When an adequate volume of test specimen is dispensed into the sample well of the test cassette, the specimen migrates by capillary action across the cassette. IgM anti-Typhoid, if present in the specimen, will bind to the Typhoid conjugates. The immunocomplex is then captured by the reagent pre-coated on the T2 band, forming a burgundy colored T2 band, indicating a Typhoid IgM positive test result and suggesting a fresh infection. IgG anti-Typhoid if present in the specimen will bind to the Typhoid conjugates. The immunocomplex is then captured by the reagent coated on the T1 band, forming a burgundy colored T1 band, indicating a Typhoid IgG positive test result and suggesting a recent or repeat infection. Absence of any T bands (T1 and T2) suggests a negative result. The test contains an internal control (C band) which should exhibit a burgundy colored band of the immunocomplex of goat anti rabbit IgG/rabbit IgG-gold conjugate regardless of the color development on any of
the T bands. Otherwise, the test result is invalid and the specimen must be retested with another device.