Product Description
REAGEN Zearalenone ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Zearalenone in cereals, feed, milk , seed and oils.
The unique features of the kit
- High recovery (>80%),rapid(10-20minutes), cost-effective extraction methods.
- High sensitivity (1ng/g or ppb).
- A quick ELISA assay (less than 1 hours regardless of number of samples)
- High reproducibility.
Procedure Overview
The method is based on a competitive colorimetric ELISA assay. The toxin of interest has been coated in the plate wells. During the analysis, sample is added along with the primary antibody specific for the target toxin and enzyme-conjugate. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the toxin attached to the well. The enzyme-conjugate, will bined with the primary antibody that is complexed to the toxin coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.
Kit Contents, Storage and Shelf Life
REAGEN Zearalenone ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C *. The shelf life is 12 months when the kit is properly stored.
| Kit Contents | Amount | Storage |
| Zearalenone-Coated Plate | 1 x 96-well Plate (8 wells x 12 strips) | 2-8°C |
| Zearalenone Standards:
Negative control (white cap tube) 0.1 ng/mL (yellow cap tube) 0.4 ng/mL (orange cap tube) 1.6 ng/mL (pink cap tube) 6.4 ng/mL (purple cap tube) 25.6 ng/mL (blue cap tube) 100 ng/mL (spiking, optional, red cap tube) |
1.0 mL 1.0 mL 1.0 mL 1.0 mL 1.0 mL 1.0 mL 1.0 mL |
2-8°C |
| Zearalenone Antibody #1 | 6mL | 2-8°C |
| HRP Conjugated Antibody #2 | 6mL | 2-8°C |
| 20X Wash Solution | 30 mL | 2-8°C |
| 10X Sample Dilution Buffer | 30 mL | 2-8°C |
| Stop Buffer | 12 mL | 2-8°C |
| TMB Substrate | 12 mL | 2-8°C |
*If you are not planning to use the kit for over 1 month, store Zearalenone Antibody #1 and HRP Conjugated Antibody #2 at -20°C or in a freezer.
Sensitivity (Detection Limit)
| Sample Type | Detection Limit (ng/g or ppb) |
| Sereal/Feed/Seed | 10 |
| Milk | 1.0 |
| Oils | 2.0 |
Specificity (Cross-Reactivity)
| Analytes | Cross-Reactivity (%) |
| Zearalenone | 100.0 |
| Zearalanone | 138 |
| α-Zearalenol | 91 |
| β-Zearalanol | 21 |
| α-Zearalanol | 69 |
| β-Zearalanol | 6 |
Reviews
There are no reviews yet.