Intended Use

The Dengue Antibody Rapid Test Kit is a rapid chromatographic immunoassay for the qualitative detection of antibodies (IgA, IgG and IgM) to dengue virus in Whole Blood /Serum/ Plasma to aid in the diagnosis of Dengue viral infection

Summary

Dengue is transmitted by the bite of an Aedes mosquito infected with any one of the four dengue viruses. It occurs in tropical and sub-tropical areas of the world. Symptoms appear 3—14 days after the infective bite. Dengue fever is a febrile illness that affects infants, young children and adults. Dengue haemorrhagic fever (fever, abdominal pain, vomiting, bleeding) is a potentially lethal complication, affecting mainly children. Early clinical diagnosis and careful clinical management by experienced physicians and nurses increase survival of patients. Dengue Antibody Rapid Test Kit is a simple, visual qualitative test that detects dengue virus antibodies in human Whole Blood/serum/plasma. The test is based on immunochromatography and can give a result within 15 minutes

Principle

The Dengue Antibody Rapid Test Kit is a qualitative membrane strip based immunoassay for thedetection of dengue virus antibodies (IgA, IgG and IgM) in Whole Blood /Serum / Plasma. Thetest device consists of: 1) a burgundy colored conjugate pad containing dengue recombinant envelope antigens conjugated with Colloid gold (dengue conjugates), 2) a nitrocellulose membrane strip containing three test lines (T1, T2and T3 lines) and a control line (C line). The T1 line is pre-coated with the antibody for the detection of IgA anti-dengue, The T2 line is pre-coated with the antibody for the detection of IgM anti-dengue, T3 line is coated with antibody for the detection of IgG anti-dengue. When an adequate volume of test specimen is dispensed into the sample well of the test cassette, the specimen migrates by capillary action across the cassette. IgG anti-dengue, if present in the specimen, will bind to the dengue conjugates. The immunocomplex is then captured by the reagent pre-coated on the T3 band, forming a burgundy colored T3 line, indicating a dengue IgG positive test result. IgM anti-dengue if present in the specimen will bind to the dengue conjugates. The immunocomplex is then captured by the