Product Description
REAGEN Erythromycin ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Erythromycin in water, urine,serum, meat(beef,chicken and pork),fish and shrimp samples.
The unique features of the kit are:
- High recovery (80-105%),rapid(less than 40 minutes) extraction methods for various samples.
- High sensitivity (0.05ng/g or ppb)and low detection limit (44ppb)for fish and Milk.
- High reproducibility..
- A quick ELISA assay(less than 2 hours regardless of number of samples).
Procedure Overview
The method is based on a competitive colorimetric ELISA assay. The drug of interest has been coated in the plate wells. During the analysis, sample is added along with the primary antibody specific for the target drug. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the drug attached to the well. The secondary antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the drug coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.
Kit Contents, Storage and Shelf Life
REAGEN Erythromycin ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C *. The shelf life is 12 months when the kit is properly stored.
| Kit Contents | Amount | Storage |
| Erythromycin Coated Plate | 1x 96-well Plate(8wells x12 strips) | 2-8°C |
| Erythromycin Standards:
Negative control (white cap tube) 0.05ng/mL(yellow cap tube) 0.15ng/mL(orange cap tube) 0.45 ng/mL (pink cap tube) 1.35 ng/mL(purple cap tube) 4.05 ng/mL (blue cap tube) 100 µg/mL (spiking, optional, red cap tube) |
1.0 mL 1.0 mL 1.0 mL 1.0 mL 1.0 mL 1.0 mL
|
2-8°C * |
| 100 ng/mL (spiking, optional, red cap tube) | 1.0 mL | |
| Erythromycin Antibody #1 | 6 mL | 2-8°C
2-8°C |
| HRP Conjugate Antibody #2 | 6 mL | |
| 20X Wash Solution | 30 mL | |
| Stop Buffer | 12 mL | |
| TMB Substrate | 12 mL | |
| Sample Extraction Buffer A | 1×5ml | |
| Sample Extraction Buffer B | 1×5ml | |
| 10X PBS | 25 mL |
* If you are not planning to use the kit for over 1 month, store Erythromycin Standards, Erythromycin Antibody and HRP Conjugate at -20°C or in a freezer.
Sensitivity (Detection Limit)
| Sample Type | Detection Limit (ppb) |
| Dirty Water | 0.2 |
| Meat/Fish/shrimp | 0.4 |
| Milk | 0.44 |
Specificity (Cross-Reactivity)
| Analytes | Cross-Reactivity (%) |
| Erythromycin | 100 |
| Erythromycin Thiocyanate | 110 |
| Erythromycin Ethylsuccinate | 68 |
| Kanamycin | < 0.1 |
| Tilmicosin | < 0.1 |
| Tylosin | < 0.1 |
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