Product Description
REAGEN Melamine ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of melamine in milk, yogurt, milk powder, egg, meat, fish, feed and other food samples.
The unique features of the kit
- High recovery (>80%),rapidand cost-effective extraction methods.
- High sensitivity(1ng/g or ppb)
- A quick ELISA assay(less than 1hours regardless of number of samples).
- High reproducibility.
Procedure Overview
REAGEN The method is based on a competitive colorimetric ELISA assay. The drug of interest has been coated in the plate wells. During the analysis, sample and HRP-Conjugated Antibody #2 are added along with the primary antibody specific for the target drug. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the drug attached to the well. At the same time, the second Antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the drug coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.
Kit Contents, Storage and Shelf Life
REAGEN Melamine ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C *. The shelf life is 12 months when the kit is properly stored.
| Kit Contents | Amount | Storage |
| Melamine Coated Plate | 1 x 96-well Plate (8 wells x 12 strips) | 2-8°C |
| Melamine Standards:
Negative control (white cap tube) 1 ng/mL (yellow cap tube) 2.5ng/mL (orange cap tube) 5 ng/mL (pink cap tube) 15ng/mL (purple cap tube) 45ng/mL (blue cap tube) 10 µg/mL (spiking, red cap tube) |
1.0 mL 1.0 mL 1.0 mL 1.0 mL 1.0 mL 1.0 mL 1.0 mL |
2-8°C |
| Melamine Antibody 1# | 6.0 mL | 2-8°C |
| HRP Conjugate Antibody 2# | 6.0 mL | 2-8°C |
| 20X Wash Solution | 30 mL | 2-8°C |
| Stop Buffer | 12 mL | 2-8°C |
| TMB Substrate | 12 mL | 2-8°C |
| 10 x Sample Extraction Buffer | 28 mL | 2-8°C |
| Melamine Sample Balance Buffer | 2 mL | 2-8°C |
| Melamine Sample Dilution Buffer I | 25 mL | 2-8°C |
| Melamine Sample Dilution Buffer II | 15 mL | 2-8°C |
| Extraction Buffer A(optional) | 5 mL | 2-8°C |
| Extraction Buffer B(optional) | 5 mL | 2-8°C |
*If you are not planning to use the opened kit for over 3 month, store Standards, Antibody and HRP Conjugate at -20°C or in a freezer.
Reviews
There are no reviews yet.