Intended Use

REAGEN The AIV-H5N 1 Antigen Rapid Test Kit is used to detect the avian influenza virus (AIV) H5N1 antigen in milk samples, and is used for the screening and auxiliary diagnosis of avian influenza virus (AIV) H5N1 infection.

Mechanism of Reaction

This kit is made based on the principle of immunochromatographic test. The sample moves along the chromatographic membrane together with the marker after being added to the sample well. If there is AIV H5N1 antigen in the sample, it will show red when it binds to the marker and the antibody on the detection line(T line), if there is no AIV H5N1 antigen in the sample, there will be no color reaction.The red band appearing in the control line area (C  line) are the criteria for determining whether there are enough samples and whether the chromatography process is normal, and also serve as the internal control standard for reagents.

Kit Contents, Storage and Shelf Life

REAGEN The AIV-H5N1 Antigen Rapid Test Kit has the capacity for 20 determinations.The shelf life is 24 months when the kit is properly stored.

Sample Preparation

1. This kit is suitable for detecting milk samples.

2. Test samples should be collected fresh and should be tested as soon as possible within 1 hour.

3. If it cannot be detected in time, milk samples can be stored at 2-8℃ for 3 days, and at –20℃ for 1 month.

Testing Protocol

1. Tear the aluminum foil bag of the test card and take out the test card, put it on a flat and clean table (pay attention to restoring the test card and reagent to room temperature before the experiment).

2. Take 3 drop of milk sample with a plastic dropper into the Sample Dilution Buffer,and mix well.

3. Drop 3 drops of mixed sample into the test card vertically, start the timer.

4. Leave it at room temperature for 10-15 minutes to judge the result, and the result after 20 minutes is invalid.

Result Interpretation

Negative: In the observation well, only a red line appeared in the control line area (C).

Positive: In the observation well, the detection line area (T) and the control line area (C) appeared red line at the same time. The color of the line can be dark or light, and all are

positive results.

Invalid: Control line fails to appear. Insufficient specimen volume or incorrect procedural techniques are the most likely reasons for control line failure. Review the procedure and

repeat the test with a new test.The following instructions are for visual interpretation only.