Kanamycin ELISA Test Kit

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Kanamycin ELISA Test Kit

$999.00

Kanamycin ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Kanamycin in meat/liver/kidney,cell lysate/culture medium/plasmid, urine, serum and milk.

Availability: 100 in stock (can be backordered)

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Product Description

REAGEN  Kanamycin ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Kanamycin in meat/liver/kidney,cell lysate/culture medium/plasmid, urine, serum and milk.

The unique features of the kit are: 

  • High recovery (>80%), rapid (10-40 minutes), and cost-effective extraction methods.
  • High sensitivity (2ng/g or ppb).
  • High reproducibility.
  • A quick ELISA assay (less than1 hours regardless of number of samples).

Procedure Overview

The method is based on a competitive colorimetric ELISA assay. The Kanamycin-BSA has been coated in the plate wells. During the analysis, sample and Kanamycin antibody (Antibody #1) and HRP-Conjugate (HRP-Conjugated Antibody #2) are added to the wells for incubation. If the Kanamycin residue is present in the sample, it will compete with the Kanamycin on the plate wells for the Kanamycin antibody. A secondary antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the drug coated on the plate wells. The resulting color intensity, after addition of the substrate (TMB), has an inverse relationship with the Kanamycin residue concentration in the sample.

Kit Contents, Storage and Shelf Life

REAGEN   Kanamycin ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C *. The shelf life is 12 months when the kit is properly stored.

Kit Contents Amount Storage
Kanamycin-coated  Plate 1 x 96-well Plate 2-8°C
Kanamycin Standards

Negative control (white CAP tube)

0.2 ng/mL (yellow CAP tube)

0.6 ng/mL (orange CAP tube)

1.8 ng/mL (pink cap tube)

5.4 ng/mL(purple cap tube)

16.2 ng/mL (blue cap tube)

1000 ng/mL(spiking, optional, red cap tube)

 

1mL

1mL

1mL

1mL

1mL

1mL

1mL

 

 

 

2-8℃

 

 

 

Kanamycin Ab#1 6.0mL 2-8℃
HRP-Conjugated Ab#2 6.0mL
10×Sample diluent 15 mL
20× Wash Solution 28mL
Stop Buffer 12mL
TMB Substrate 12mL
10×Sample Ext. buffer (optional) 15mL
Sample Balance Buffer (optional) 2 mL

* If you are not planning to use the kit for over 1 month, store Kanamycin Standard Stock, Kanamycin Antibody #1and  HRP-Conjugated Antibody #2 at -20°C or in a freezer.

Sensitivity (Detection Limit)

Sample Type Detection Limit (ng/g or ppb)
Fish/Shrimp/Meat/liver/kidney 8
Cell Lysate/Culture medium/Plasmid 2
Urine /Serum 5
Milk 2

Specificity (Cross-Reactivity)

Analytes Cross-Reactivity (%)
Kanamycin 100
Streptomycin < 0.1
Dihydrostreptomycin < 0.1
Neomycin < 0.1
Brand

REAGEN

Model

RND99033

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IMG_20230823_112650Kanamycin ELISA Test Kit
$999.00

Availability: 100 in stock (can be backordered)