Product Description
REAGEN Streptomycin ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of streptomycin and dihydrostreptomycin in feed, honey, kidney, liver, meat (beef, chicken and pork), milk, milk powder ,serum and urine.
The unique features of the kit are:
- Rapid (10 – 30 minutes), and organic reagent-free extraction methods for various samples with high recovery (75- 115%).
- High sensitivity (0.1ng/g or ppb) and low detection limit (1 ng/g or ppb for meat and milk).
- High reproducibility.
- A quick ELISA assay ( 1hours regardless of number of samples).
Procedure Overview
REAGEN The method is based on a competitive colorimetric ELISA assay. The streptomycin has been coated in the plate wells. During the analysis, sample is added along with the HRP-Conjugated Antibody #2 and Streptomycin Antibody #1 . If the streptomycin residue is present in the sample, it will compete for the streptomycin antibody #1, thereby preventing the streptomycin from binding to the antibody attached to the well. The resulting color intensity, after addition of the HRP substrate (TMB), has an inverse relationship with the streptomycin residue concentration in the sample.
Kit Contents, Storage and Shelf Life
REAGEN Streptomycin ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C *. The shelf life is 12 months when the kit is properly stored.
