Product Description
REAGENĀ Ā Natamycin ELISA Test Kit enables government agencies, food manufacturers and quality assurance organizations to detect Natamycin to as low as 0.2 ppm level in various sample types and to satisfy customer concerns about food safety.
The unique features of the kit
- The extraction of Natamycin from meat/tissue with a high recovery rate.
- A quick ELISA assay (less than 1 hours regardless of number of samples).
- High reproducibility Procedure Overview.
ProcedureĀ Overview
The method is based on a competitive colorimetric ELISA assay. The drug of interest has been coated in the plate wells. During the analysis, sample andĀ HRP-Conjugated Antibody #2Ā are added along with the primary antibody specific for the target drug. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the drug attached to the well. At the same time, the second Antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the drug coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.
Kit Contents, StorageĀ and Shelf Life
REAGENĀ Ā NatamycinĀ ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8Ā°C . The shelf life is 12 months when the kit is properly stored.
| Kit Contents | Amount | Storage |
| NatamycinĀ Coated Plate | 1 x 96-well Plate (8 wells x 12 strips) | 2-8Ā°C |
| NatamycinĀ Standards:
Negative control (white cap tube) 0.1Ā ug/mLĀ (yellow cap tube) 0.25 ug/mL (orange cap tube) 0.5 ug/mL (pink cap tube) 1.0 ug/mL (purple cap tube) 5.0 ug/mL (blue cap tube) 100ug/mLĀ (spiking, optional, red cap tube) |
1.0Ā mL 1.0Ā mL 1.0Ā mL 1.0Ā mL 1.0Ā mL 1.0Ā mL 1.0Ā mL |
2-8Ā°C |
| 10 XĀ NatamycinĀ Antibody 1# | 0.8Ā mL | 2-8Ā°C |
| 10 XĀ HRP Conjugate Antibody 2# | 0.8 mL | 2-8Ā°C |
| Antibody Solution | 16 mL | 2-8Ā°C |
| 3Ā X Sample Dilution Bufferļ¼optionalļ¼ | 30 mL | 2-8Ā°C |
| 10 X Sample Extraction Buffer | 30 mL | 2-8Ā°C |
| 20X Wash Solution | 30 mL | 2-8Ā°C |
| TMB Substrate | 12 mL | 2-8Ā°C |
| Stop Buffer | 12 mL | 2-8Ā°C |
*If you are not planning to use the kit for over 1 month, store Ā NatamycinĀ Ā Ā Ā Ā Standards, NatamycinĀ Antibody and Ā HRP-Conjugate at -20Ā°C or in a freezer.
Sensitivity (Detection Limit)
| Sample Type | Detection Limit (ng/g or ppb) |
| Fresh Milk | 0.2 |
| Egg/Pure Milk/Urine | 1 |
| Milk powder/Infant Formula | 2 |
| Meat/TissueĀ /Fish/Shrimp | 0.2 |
Specificity (Cross-Reactivity)
| Analytes | Cross-Reactivity (%) |
| Natamycin | 100 |
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