Furaltadone(AMOZ) ELISA Test Kit

,

Furaltadone(AMOZ) ELISA Test Kit

Furaltadone(AMOZ) ELISA Test Kit provides a competitive enzyme immunoassay for the quantitative analysis of furaltadone in fish, shrimp, meat (chicken,beef,pork andĀ hepar), eggs, hone.

Product Description

REAGENĀ Ā Furaltadone(AMOZ) ELISA Test Kit provides a competitive enzyme immunoassay for the quantitative analysis of furaltadone in fish, shrimp, meat (chicken,beef,pork andĀ hepar), eggs, hone.

  • Rapid (4 hours), high recovery (80 – 105%), and cost-effective extraction methods for various samples.
  • High sensitivity (0.05ng/g or ppb) and low detection limit (0.1Ā ng/g or ppb) for various samples.
  • High reproducibility.
  • A quick ELISA assay (less than 1hours regardless of number of samples).

ProcedureĀ Overview

The method is based on a competitive colorimetric ELISA assay. The AMOZ-BSA has been coated in the plate wells. During the analysis, sample and the AMOZ antibody areĀ added along with secondary antibody, tagged with a peroxidase enzyme.Ā If the AMOZĀ residue is present in the sample, it will compete for the AMOZĀ antibody, thereby preventing the antibody from binding to theĀ AMOZ-BSAĀ attached to the well. The resulting color intensity, after addition of the HRP substrate (TMB), has an inverse relationship with the AMOZĀ residue concentration in the sample.

Kit Contents, StorageĀ and Shelf Life

REAGENĀ Ā Furaltadone (AMOZ) ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8Ā°C*. The shelf life is 12 months when the kit is properly stored.

Kit Contents Amount Storage
AMOZĀ -coatedĀ Plate 1 x 96-well plate (8 wells x 12 strips) 2-8Ā°C
AMOZ Standards:

Negative control (white cap tube)

0.0Ā 5ng/mL (yellow cap tube)

0.15ng/mL (orange cap tube)

0.45Ā ng/mL (pink cap tube)

1.35Ā ng/mL (purple cap tube)

4.05Ā ng/mL (blue cap tube)

10 ng/mL (spiking, optional, red cap tube)

 

1.5Ā mL

1.5Ā mL

1.5Ā mL

1.5Ā mL

1.5Ā mL

1.5Ā mL

1.5Ā mL

2-8Ā°C

 

 

 

 

2-8Ā°C

AMOZ AntibodyĀ (Antibody#1) 4 mL
HRP-Conjugated Antibody #2 6 mL 2-8Ā°C
10X Sample Extraction Buffer 25 mL
20X Wash Solution 28Ā mL
Stop Buffer 20 mL
TMB Substrate 12Ā mL
50 mM 2-Nitrobenzaldehyde 2.0Ā mL

*Ā If you are not planning to use the kit for over 1 month, store Antibody#1 and HRP-Conjugated Antibody #2Ā at -20Ā°CĀ or in a freezer.

Sensitivity (Detection Limit)

Sample Type Detection Limit (ng/g or ppb)
Fish/Shrimp 0.1
Meat (chicken,beef,porkĀ and hepar) 0.1
Egg/Egg Powder 0.1
Honey 0.1

Specificity (Cross-Reactivity)

Analytes Cross-ReactivityĀ (%)
AMOZ 100
AOZ <0.04
AHD < 0.06
SEM < 0.05

Brand

REAGEN

Model

RND99012

Reviews

There are no reviews yet.

Be the first to review “Furaltadone(AMOZ) ELISA Test Kit”

Your email address will not be published. Required fields are marked *