Product Description
REAGEN Oxytetracycline ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Oxytetracycline in in honey, butter, fermented milk, feed, egg, cheese, milk, meat, meat products (sausage), fish and shrimp.
The unique features of the kit are:
- High recovery (70-120%), rapid (less than 30 minutes), and cost-effective extraction methods.
- High reproducibility.
- A quick ELISA assay (less than 2 hours regardless of number of samples).
Procedure Overview
REAGEN Oxytetracycline ELISA Test Kit is based on a competitive colorimetric ELISA assay. The drug of interest has been coated in the plate wells. During the analysis, sample is added along with the primary antibody specific for the target drug. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the drug attached to the well. The secondary antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the drug coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.
Kit Contents, Storage and Shelf Life
REAGEN Oxytetracycline ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C . The shelf life is 12 months when the kit is properly stored.
| Kit Contents | Amount | Storage |
| Oxytetracycline-Coated Plate | 1 x 96-well plate (8 wells x 12 strips) | 2-8℃ |
| Oxytetracycline Standards:
Negative control (white Cap tube) 3ng/mL (yellow Cap tube) 6 ng/mL (orange Cap tube) 12 ng/mL (pink Cap tube) 24 ng/mL (purple Cap tube) 96 ng/mL (blue Cap tube) 100ng/mL (Spiking red Cap tube) |
0.2 mL 0.2 mL 0.2 mL 0.2 mL 0.2 mL 0.2 mL 1 mL |
2-8℃ |
| Oxytetracycline Antibody #1 | 6mL | 2-8℃ |
| HRP-Conjugated Antibody #2 | 12mL | |
| 20X Wash Solution | 30mL | |
| Stop Buffer | 12mL | |
| 30X Sample Dilution | 30mL | |
| TMB Substrate | 12mL |
* If you are not planning to use the kit for over 1 month, storing Oxytetracycline Standard Stock, Oxytetracycline Antibody #1 and HRP-Conjugated Antibody #2 at －20℃ or in a freezer is recommended.
Detection Limit Of Quantitation
| Sample Type | Limit Of Quantitation (ng/g) |
| Egg/Cheese/Fermented Milk | 1.2 |
| Honey | 1.2 |
| Meat/Meat Products/Fish/Shrimp/Butter | 2.4 |
| Milk Powder | 3 |
| Yogurt, Fresh Milk, Condensed Milk | 3 |
| Feed | 15 |
Specificity (Cross-Reactivity)
| Analytes | Cross-Reactivity (%) |
| Oxytetracycline | 100 |
| Chlortetracycline
|
99 |
| Tetracycline | 46 |
| Doxycycline | 58 |
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