Prednisolone ELISA Test Kit


Prednisolone ELISA Test Kit

Prednisolone ELISA Test Kit is a competitive enzyme immunoassay for the detection of Prednisolone in meat, liver,and milk.

Product Description

REAGEN  Prednisolone ELISA Test Kit is a competitive enzyme immunoassay for the detection of Prednisolone in meat, liver,and milk.

The unique features of the kit are:

  • High recovery (>75%), rapid, and cost-effective extraction methods
  • High sensitivity (0.05 ng/g or ppb) and low detection limit (0.11ng/g or ppb) for milk samples
  • Rapid ELISA assay (less than 2 hours regardless of number of samples)
  • Highreproducibility

Procedure Overview

REAGEN  The test is based on a competitive colorimetric ELISA assay. The drug of interest has been coated in the plate wells. During analysis, sample is added along with the primary antibody specific for the target drug. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the drug attached to the well. The secondary antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the drug coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.

Kit Contents, Storage and Shelf Life

REAGEN  Prednisolone ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C . The shelf life is 12 months when the kit is properly stored.

Kit Contents Amount Storage
Prednisolone-Coated Plate 1 x 96-well plate (8 wells x 12 strips) 2-8℃
Prednisolone Standards:

Negative control

0.1 ng/mL

0.4 ng/mL

1.6 ng/mL

6.4 ng/mL

25.6 ng/mL

100 ng/mL(spiking, optional)
















Prednisolone Antibody #1 6mL 2-8℃
HRP-Conjugated Antibody #2 12mL
20× Wash Solution 30mL
Stop Buffer 12mL
TMB Substrate 12mL
10X Sample Dilution Buffer 25mL
Sample Extraction Buffer A 5 mL
Sample Extraction Buffer B 5 mL

* If you are not planning to use the kit for over 1 month ,Prednisolone Antibody #1 and 100× HRP-Conjugated Antibody #2 at -20℃ or in a freezer is recommended.

Detection Limit of Quantitation

Sample Type Detection Limit (ng/g or ppb)
Milk 0.11
milk powder 0.11
Meat/Liver 0.15

Specificity (Cross-Reactivity)

Analytes Cross-Reactivity (%)
Prednisolone 100






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