Product Description

REAGEN   Trifluralin ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of trifluralin in water, fish, shrimp and meat. Trifluralin can persist in soils for many months after it is used and is highly toxic to aquatic animals (fish and invertebrates). Sediment-feeding organisms are particularly at risk because of the tendency of trifluralin to bioaccumulate. Studies also suggest exposure-related abnormalities in the vertebral development of aquatic animals, at low concentrations.

The unique features of the kit are:

• High recovery (75- 125%).
• High sensitivity (1ng/g ) and low detection limit (4 ng/g) forfish/shrimp
• High reproducibility.
• A quick ELISA assay.

Procedure Overview

The method is based on a competitive colorimetric ELISA assay. The drug of interest has been coated in the plate wells. During the analysis, sample is added along with the primary antibody specific for the target and enzyme-conjugate. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the drug attached to the well. The enzyme-conjugate, will bined with the primary antibody that is complexed to the drug coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.

Kit Contents, Storage and Shelf Life

REAGEN  Trifluralin ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused micro-wells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C *. The shelf life is 12 months when the kit is properly stored.

Detection Limit Of Quantitation

Specificity (Cross-Reactivity)