Product Description

Acrylamide ELISA is an immunoassay for the detection of Acrylamide. This test is suitable for the quantitative detection of Acrylamide in fried food (e.g. potato chips/chips).

Procedure Overview

The method is based on a competitive colorimetric ELISA assay. The drug of interest has been coated in the plate wells. During the analysis, sample is added along with the primary antibody specific for the target drug. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the drug attached to the well. The secondary antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the drug coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.

Possible Test Interference

Numerous organic and inorganic compounds commonly found in samples have been tested and found not to interfere with this test. However, due to the high variability of compounds that might be found in samples, test interferences caused by matrix effects cannot be completely excluded.

Mistakes in handling the test can also cause errors. Possible sources for such errors can be: Inadequate storage conditions of the test kit, incorrect pipetting sequence or inaccurate volumes of the reagents, too long or too short incubation times during the immune and/or substrate reaction, extreme temperatures during the test performance (lower than 2°C or higher than 8°C during the first incubation or lower than 10°C or higher than 30°C during the color incubation step), inappropriate sample clean up or derivatization.