Product Description
REAGEN Ractopamine ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Ractopamine and other related ß-agonists in feed, tissue, milk, milk powder and egg. Ractopamine ELISA Test Kit enables international and government regulatory agencies, food manufacturers and processors, as well as quality assurance organizations, to detect Ractopamine in various sample types in response to customer concerns about food safety.
The unique features of the kit are:
- Rapid (10 – 30 minutes), and organic reagent-free extraction method for various samples withhigh recovery 70 – 120%).
- High sensitivity (0.05 ng/g or ppb) and low detection limit (0.5ng/g or ppb for meat/tissue, 0.8 ng/g or ppb for Milk and 5 ng/g or ppb for feed).
- A quick ELISA assay (less than 5hours regardless of number of samples.
- High reproducibility.
Procedure Overview
REAGEN Ractopamine The method is based on a competitive colorimetric ELISA assay. The drug of interest has been coated in the plate wells. During the analysis, sample and HRP-Conjugated Antibody #2 are added along with the primary antibody specific for the target drug. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the drug attached to the well. At the same time, the second Antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the drug coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.
Kit Contents, Storage and Shelf Life
| Kit Contents | Amount | Storage |
| Ractopamine-coated Microtiter Plate | 1 x 96-well plate (8 wells x 12 strips) | 2-8°C |
| Ractopamine Standards:
Negative control (white cap tube) 0.04 ng/mL (yellow cap tube) 0.1 ng/mL (orange cap tube) 0.25 ng/mL (pink cap tube) 0.6 ng/mL (purple cap tube) 1.5 ng/mL (blue cap tube) 100 ng/mL (spiking, optional, red cap tube) |
1 mL 1 mL 1 mL 1 mL 1 mL 1 mL 1 mL |
2-8°C |
| Ractopamine Antibody #1 | 6 mL | 2-8℃ |
| HRP-Conjugated Antibody#2 | 6 mL | |
| 10X Sample Dilution Buffer | 30 mL | |
| 20X Wash Solution | 30 mL | |
| Stop Buffer | 12 mL | |
| TMB Substrate | 12 mL |
* If you are not planning to use the kit for over 1 month, store HRP-Conjugated at -20°C or in a freezer.
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