Ampicillin ELISA Test Kit

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Ampicillin ELISA Test Kit

Ampicillin ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Ampicillins in milk, urine, fish, tissue (liver, kidney, and muscle), serum, and plasma samples. Ampicillin ELISA Test Kit enables international and government regulatory agencies, food manufacturers and processors, as well as quality assurance organizations, to detect Ampicillin containing antibiotics to satisfy customer concerns about food safety.

Availability: 100 in stock (can be backordered)

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Product Description

REAGEN  Ampicillin ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Ampicillins in milk, urine, fish, tissue (liver, kidney, and muscle), serum, and plasma samples. Ampicillin ELISA Test Kit enables international and government regulatory agencies, food manufacturers and processors, as well as quality assurance organizations, to detect Ampicillin containing antibiotics to satisfy customer concerns about food safety.

The unique features of the kit are:

  • High recovery (75-105%), low-cost rapid sample prep protocol (10 minutes – 1 hour)
  • High sensitivity: 15ng/g = 0.15 ppb
  • High reproducibility (interplate and intraplate CV typically <8%)
  • Rapid assay protocol (less than 2 hours to process 40 samples in duplicate)

Procedure Overview

REAGEN  The method is based on a competitive colorimetric ELISA assay. The drug of interest (Ampicillin antibiotic) has been coated in the plate wells. The sample to be tested is mixed with a high-affinity Ampicillin capture protein and added to the well. If the Ampicillin target is present in the sample, it will compete for the capture protein, thereby preventing the capture protein from binding to the drug attached to the well. The secondary antibody, tagged with a peroxidase enzyme, targets the capture protein bound to the drug coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample. The levels of Ampicillin antibiotic in the sample are determined by comparison of the color intensity of unknown samples with the standard curve, which is made using the Ampicillin standard provided in the kit.

Kit Contents, Storage and Shelf Life

Kit Contents Amount Storage
Ampicillin Coated Plate 1 x 96-well Plate

(8 wells x 12 strips)

2-8°C
Ampicillin Powder stock (1000 ng):

Empty Vials for Standards:

Negative control (white cap tube)

0.15 ng/mL (yellow cap tube)

0.3 ng/mL (orange cap tube)

0.6ng/mL (pink cap tube)

1.8 ng/mL(purple cap tube)

5 .4ng/mL (blue cap tube)

2

 

1

1

1

1

1

1

2-8°C *
 Ampicillin Capture Protein 6 mL 2-8°C *
 Ampicillin Capture Protein Antibody #1 6 mL
HRP Conjugate Antibody 2# 6 mL
20X Wash Solution 30 mL
Stop Buffer 12 mL
10X Ampicillin Extraction Buffer 30 mL
TMB Substrate 12 mL

* If the kit is to be stored for over two weeks before use, store Capture Protein Antibody #1, Ampicillin Powder stock, Ampicillin Capture Protein and HRP-Conjugated Antibody #2 at -20°C in a freezer that does not cycle to higher temperatures (i.e. a non-frost-free freezer). To avoid repeated freeze-thawing of the capture protein it is strongly recommended to store this component in single-use aliquots.

Sensitivity (Detection Limit)

 

Antibiotic Milk, Meat, Tissue, Fish and Urine(ppb) Serum and Plasma(ppb)
Ampicillin 1.5 3

 Specificity (Cross-Reactivity)

Analytes Cross-Reactivity (%)
Ampicillin 100
Penicillin G 125
Azlocillin 112
Piperacillin 70
Nafcillin 55
Amoxicillin 44
Cloxacillin 40
Oxacillin 40
Cefoperazone 34
Ceftiofur 4

 

Brand

REAGEN

Model

RND99091

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IMG_20230823_112650Ampicillin ELISA Test Kit
$999.00

Availability: 100 in stock (can be backordered)