Clenbuterol ELISA Test Kit

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Clenbuterol ELISA Test Kit

$999.00

Clenbuterol ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of clenbuterol in feed, liver, tissue and urine. 

Availability: 100 in stock (can be backordered)

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Product Description

REAGEN  Clenbuterol ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of clenbuterol in feed, liver, tissue and urine. 

Clenbuterol ELISA Test Kit enables international and government regulatory agencies, food manufacturers and processors, as well as quality assurance organizations, to detect clenbuterol in various sample types in response to customer concerns about food safety.

The unique features of the kit are:

  • Rapid (10 – 30 minutes), and organic reagent-free extraction method for various samples withhigh recovery (75 – 95%).
  • High sensitivity (0.05 ng/g or ppb)
  • A quick ELISA assay (less than 5hours regardless of number of samples.
  • High reproducibility.

Procedure Overview

The method is based on a competitive colorimetric ELISA assay. The plate wells are coated with antigen.During the analysis, sample is added along with the HRP-Conjugated. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the drug attached to the well. The secondary antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the drug coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.

Kit Contents, Storage and Shelf Life

Kit Contents Amount Storage
Clenbuterol-coated Microtiter Plate 1 x 96-well plate (8 wells x 12 strips) 2-8°C
Clenbuterol Standards:

Negative control

0.05 ng/mL

0.15 ng/mL

0.45 ng/mL

1.35 ng/mL

4.05 ng/mL

100 ng/mL (spiking, optional,)

 

1 mL

1 mL

1 mL

1 mL

1 mL

1 mL

1 mL

2-8°C
Clenbuterol Antibody #1 5.5mL
HRP-Conjugated Antibody #2 5.5mL 2-8℃
10X Sample Extraction Buffer ** 50 mL
20X Wash Solution ** 40 mL
Stop Buffer ** 6 mL
TMB Substrate ** 12 mL

* If you are not planning to use the kit for over 1 month, store Antibody #1,  HRP-Conjugated at -20°C or in a freezer.

Sensitivity (Detection Limit)

Sample Type Detection Limit (ng/g or ppb)
Feed 1
Tissue 0.2
Urine 0.1
Liver 0.5

Specificity (Cross-Reactivity)

Analytes Cross-Reactivity (%)
Clenbuterol 100
Mabuterol 62
Tolubuterol 12
Terbutaline 8.3
Cimbuterol 6.7
Salbutamol 6.0
Cimaterol 1.6
Pirbuterol 0.5
Brand

REAGEN

Model

RND99013

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IMG_20230915_174149Clenbuterol ELISA Test Kit
$999.00

Availability: 100 in stock (can be backordered)