Benzo[a]pyrene (BAP) ELISA Test Kit

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Benzo[a]pyrene (BAP) ELISA Test Kit

$999.00

Benzo[a]pyrene (BAP) ELISA Test Kit provides a competitive enzyme immunoassay for the quantitative analysis of Benzo[a]pyrene in water and vegetable oil .

Availability: 100 in stock (can be backordered)

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Product Description

REAGEN Benzo[a]pyrene (BAP) ELISA Test Kit provides a competitive enzyme immunoassay for the quantitative analysis of Benzo[a]pyrene in water and vegetable oil .

The unique features of the kit are:

  • Rapid and cost-effective extraction methods for various samples.
  • High sensitivity (0.04ng/g or ppb)
  • A quick ELISA assay (less than 2 hours regardless of number of samples).

Procedure Overview

The method is based on a competitive colorimetric ELISA assay. The drug of interest has been coated in the plate wells. During the analysis, sample is added along with the primary antibody specific for the target drug. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the drug attached to the well. The secondary antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the drug coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.

Kit Contents, Storage and Shelf Life

REAGEN  BaP ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C *. The shelf life is 12 months when the kit is properly stored.

Kit Contents Amount Storage
BAP-coated Microtiter Plate 1 x 96-well plate (8 wells x 12 strips) 2-8°C
200PPb BAP Standard 0.5 mL 2-8°C
Empty Vials for Standards 6 2-8°C
Standards Dilution Buffer 28mL×2 2-8°C
BAP Antibody #1 6mL 2-8°C
HRP-Conjugated Ab #2 11 mL 2-8°C
10×PBS 15mL 2-8°C
Sample Extraction Buffer 25 mL×2 2-8°C
20X Wash Solution ** 28 mL 2-8°C
Stop Buffer 12 mL 2-8°C
TMB Substrate 12 mL 2-8°C

Sensitivity (Detection Limit)

Sample Type Detection Limit (ng/g or ppb)
Vegetable Oil 2
Water 0.4

Specificity (Cross-Reactivity)

Analytes Cross-Reactivity (%)
Benzo[a]pyrene 100
Hexachlorobenzene <0.1
Hexachlorocyclopentadine < 0.1
Methoxychlor < 0.1
Brand

REAGEN

Model

RNC95006

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Product ImageBenzo[a]pyrene (BAP) ELISA Test Kit
$999.00

Availability: 100 in stock (can be backordered)