Product Description

REAGEN  Vitamin B5 Test Kit is quantitative analysis of total Vitamin B5 in food ,feed and drug.

The unique features of the kit are:

• rapid, and cost-effective extraction methods,the same as other Vitamin.
• The kit provide positive control and serial standards
• High sensitivity (80μg/100g)
• High recovery（80%-120%）
• High reproducibility(Intra-assay CV for standards: < 12%,Inter-assay CV for standards: < 15 %

Procedure Overview

REAGEN  Sample and Vitamin B5 culture medium add to the 96-microwell which has the Lactobacillus plantarum, if the sample has Vitamin B5 the Lactobacillus plantarum will grow, after culture 37℃,44-48h, read the OD by microplate reader 610 – 630 nm (or540 – 550 nm),The sample of the turbidity compared with standard curve can get Vitamin B5 content.

Kit Contents, Storage and Shelf Life

REAGEN  Vitamin B5 Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8℃ .The shelf life is 6 months when the kit is properly stored.