Product Description

REAGEN  Vitamin B7 Test Kit is quantitative analysis of total Vitamin B7 in food ,feed and drug.

 The unique features of the kit are:

• rapid, and cost-effective extraction methods,the same as other Vitamin.
• The kit provide positive control and serial standards
• High sensitivity (0.025μg/100g)
• High recovery（90%-105%）
• High reproducibility

Procedure Overview

REAGEN  Sample and Vitamin B7 culture medium add to the 96-microwell which has the Lactobacillus plantarum subsp. lactis （leichmannii）,if the sample hasVitamin B7 the Lactobacillus plantarum will grow,after culture 37℃,44-48h, read the OD by microplate reader 610 – 630 nm (or540 – 550 nm),The sample of the turbidity compared with standard curve can get Biotin content.

Kit Contents, Storage and Shelf Life

REAGEN  Vitamin B7 Test Kit has the capacity for 96 determinations or testing of 26 samples in duplicate (assuming 18 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8℃ .The shelf life is 6 months when the kit is properly stored.

Required Materials Not Provided With the Kit

• Laminar flow bench
• Microtiter plate reader：610 – 630 nm （540 – 550 nm）
• Incubator：37 °C
• Water bath 95℃
• Centrifuge：8000g
• Sterile Pipette 20-200ul,100-1000ul
• Sterile centrifuge tubes (1.5-2ml  ,15 and 50 mL)
• The 500 mL glass bottles and flasks (100 and 1000 mL), 100 mL beaker
• Sterile syringe, 0.2 um sterile membrane
• Sterile water ordeionized water
• Amylasez：B.Fluka 86250
• H2SO4：1mol/L
• NaOH concentrate(take 40g NaOH add 100ml Sterile water or deionized water)and 2mol/L NaOH(take 8g NaOH add 100ml Sterile water or deionized water)

• NaOH：1mol/L and 0.1mol/L
• HCl：1mol/L and 0.1mol/L
• Citrate buffer（pH5）,take 1.5 g citric acid monohydrate to 100 ml beaker , add 50 ml sterile water or deionized water, stirring and dissolved, then add 12 ml NaOH (1 mol/L) or 0.48 g NaOH, using 0.1 mol/L HCl to pH to 4.5;With sterile water or deionized water transferring quantitative solution into 100 ml volumetric flask, with sterile water or deionized water constant volume, can be stored three days in 2-8 ℃.