Crystal Violet/LCV ELISA Test Kit


Crystal Violet/LCV ELISA Test Kit

Crystal Violet/LCV plate kit is a competitive ELISA for the quantitative analysis of Crystal Violet/LCV in fish/shrimp.

Product Description

REAGEN  Crystal Violet/LCV plate kit is a competitive ELISA for the quantitative analysis of Crystal Violet/LCV in fish/shrimp.

The unique features of the kit are:

  • High, reproducible recovery rates.
  • Converts LeucoCrystal Violet/LCV(LMG) to Crystal Violet/LCV before detection. Chromatic Crystal Violet/LCV can be rapidly bio-converted into colorless LMG in fish. A unique oxidant solution in the kit converts LMG to the Crystal Violet/LCV form detected by the kit.
  • 96-well high-throughput quantitative detection method. The detection limit is 0.1ng/g or ppb for fish/shrimp samples.
  • Final samples can be directly used for liquid chromatography confirmation.

Procedure Overview

REAGEN  The method is based on a competitive colorimetric ELISA assay. The Crystal Violet (CV) antigen has been coated in the plate wells. During the analysis, sample is added along with the primary antibody specific for target toxin. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the toxin attached to the well. The second antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the toxin coated on the wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.

Kit Contents, Storage and Shelf Life

REAGEN  Crystal Violet/LCV ELISA Test Kit has the Crystal Violet/LCV acity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C *. The shelf life is 12 months when the kit is properly stored.

Kit Contents Amount Storage
Crystal Violet/LCV -Coated Plate 1 x 96-well Plate (8 wells x 12 strips) 2-8°C
Standard(Empty bottle):
Negative Control0.025ppb












 Spiking (10ppb) 1ml 2-8°C
Antibody #1 5.5 ml 2-8°C
HRP-Conjugated Antibody #2 11ml 2-8°C
20X Wash Solution ** 28 mL 2-8°C
Stop Buffer ** 6 mL 2-8°C
TMB Substrate ** 12 mL 2-8°C
10X Sample Dilution Buffer 20 ml 2-8°C
Oxidant Solution 6 ml 2-8°C
Cosolvent Solution 6 ml 2-8°C

* If you are not planning to use the kit for over 3 months, store Crystal Violet/LCV  Antibody #1 and HRP-Conjugated Antibody #2 at -20°C or in a freezer.


Sample Type Detection Limit (ng/g or ppb)
Fish/Shrimp 0.1


Analytes Cross-Reactivity (%)
Crystal Violet 100
Malachite Green 80
Leucocrystal Violet 100 (after oxidation)
Leucomalachite Green 80 (after oxidation)





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