Product Description
REAGEN Norfloxacin ELISA Test Kit enables government agencies, food manufacturers and quality assurance organizations to detect norfloxacin in various sample types and to satisfy customer concerns about food safety.
The unique features of the kit are:
- The extraction of norfloxacin from feed, honey, meat, milk, tissue, serum and urine can be finished in 10 – 30 minutes with a high recovery rate of 75 – 95%.
- A quick ELISA assay (less than 2 hours regardless of number of samples).
- High reproducibility.
Procedure Overview
The method is based on a competitive colorimetric ELISA assay. The norfloxacin antibody has been coated in the plate wells. During the analysis, sample is added along with the norfloxacin-horseradish peroxidase (Norfloxacin-HRP) conjugate. If the norfloxacin residue is present in the sample, it will compete for the norfloxacin antibody, thereby preventing the Norfloxacin-HRP from binding to the antibody attached to the well. The resulting color intensity, after addition of the HRP substrate (TMB), has an inverse relationship with the Norfloxacin residue concentration in the sample.
Kit Contents, Storage and Shelf Life
REAGEN Norfloxacin ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C. The shelf life is 12 months when the kit is properly stored.
| Kit Contents | Amount | Storage |
| Norfloxacin Ab-coated Plate | 1 x 96-well plate (8 wells x 12 strips) | 2-8°C |
| Norfloxacin Standards:
Negative control (white cap tube) 0.1 ng/mL (yellow cap tube) 0.25 ng/mL (orange cap tube) 0.6 ng/mL (pink cap tube) 1.5 ng/mL (purple cap tube) 5.0 ng/mL (blue cap tube) 1000 ng/mL (spiking, optional, red cap tube) |
1.8 mL 1.8 mL 1.8 mL 1.8 mL 1.8 mL 1.8 mL 1.8 mL
|
2-8°C |
| Norfloxacin-HRP Conjugate | 6 mL | 2-8°C |
| 10X Sample Extraction Buffer ** | 25 mL | |
| 20X Wash Solution ** | 28 mL | |
| Stop Buffer ** | 14 mL | |
| TMB Substrate ** | 12 mL |
*If you are not planning to use the kit for over 3 months, store HRP-Conjugated at -20°C preferably or in a freezer.
Sensitivity (Detection Limit)
| Sample Type | Detection Limit (ng/g or ppb) |
| Feed | 1 |
| Honey | 2 |
| Meat/Liver/Kidney/Fish/Shrimp | 1 |
| Milk | 0.5 |
| Serum | 0.5 |
| Urine | 0.5 |
Specificity (Cross-Reactivity)
| Fluoroquinolines | Cross-Reactivity (%) |
| Norfloxacin | 100 |
| Oxolinic acid | 40 |
| Pipemidic acid | 18 |
| Ofloxacin | 17 |
| Ciprofloxacin | 9 |
| Enrofloxacin | 6 |
| Flumequin | 6 |
| Cinoxacin | 1 |
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