Diethylstilbestrol ELISA Test Kit

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Diethylstilbestrol ELISA Test Kit

Diethylstilbestrol ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of diethylstilbestrol in fish,shrimp, meat, milk and urine.

Product Description

REAGEN  Diethylstilbestrol ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of diethylstilbestrol in fish,shrimp, meat, milk and urine.

The unique features of the kit are:

  • High recovery (80%-120%),rapid, cost-effective extraction methods.
  • High sensitivity(0.5 ng/g or ppb) andlimit of quantitation (meat/fish/shrimp 1 ppb and milk 0.75ppb).
  • A quick ELISA assay (less than 1hours regardless of number of samples).
  • High reproducibility.

Procedure Overview

The method is based on a competitive colorimetric ELISA assay. The DES-BSA has been coated in the plate wells. During the analysis, sample and DES antibody (Antibody #1) and HRP-Conjugate (HRP-Conjugated Antibody #2) are added to the wells for incubation. If the DES residue is present in the sample, it will compete with the DES on the plate wells for the DES antibody, The secondary antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the drug coated on the plate wells. The resulting color intensity, after addition of the HRP substrate (TMB), has an inverse relationship with the DES residue concentration in the sample.

Kit Contents, Storage and Shelf Life

REAGEN  Diethylstilbestrol ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C *. The shelf life is 12 months when the kit is properly stored.

Kit Contents Amount Storage
Diethylstilbestrol Coated Plate 96-well Plate (8wells x 12strips) 2-8°C
Diethylstilbestrol Standards:

Negative control (white cap tube)

0.5ng/mL (yellow cap tube)

1.0ng/mL (orange cap tube)

2.0 ng/mL (pink cap tube)

4.0ng/mL (purple cap tube)

8.0 ng/mL (blue cap tube)

100 ng/mL (spiking, red cap tube)

 

0.8 mL

0.8 mL

0.8 mL

0.8 mL

0.8 mL

0.8 mL

0.8 mL

2-8°C
Diethylstilbestrol Antibody #1 6mL 2-8°C *
 HRP-Conjugated Antibody #2 6mL 2-8°C
20X Wash Solution ** 28 mL
Stop Buffer ** 12mL
TMB Substrate ** 12 mL
10X PBS 12mL

*    If you are not planning to use the kit for over 3 months, store Diethylstilbestrol Standards, Antibody #1 and HRP-Conjugated Antibody #2 at -20°C or in a freezer.

Sensitivity (Quantitation Limit)

Sample Type Quantitation Limit (ng/g or ppb)
Meat/fish/Shrimp 1.0
Milk 0.75
Urine 2.5

Specificity (Cross-Reactivity)

Analytes Cross-Reactivity (%)
Diethylstilbestrol 100
DES-glucuronide 100
Hexestrol 100
Dienestrol 80
DES-dipropionate 50
Estradiol 45
Testerone <5
Salmeterol <5
Ractopamine <0.01
Brand

REAGEN

Model

RNH94011

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