Product Description
REAGEN Zeranol ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Zeranol in meat/fish, cereals, feed, milk , plasma, urine and seed .Zeranol (α-Zearalanol) is a non-steroidal estrogen agonist. It is a mycotoxin, derived from fungi in the Fusarium family, and may be found as a contaminant in fungus-infected crops. Zeranol is approved for use as a growth promoter in livestock, including beef cattle, in the United States. However, its application is not approved for use in the European Union or Canada.
REAGEN Zeranol ELISA Test Kit enables international and government regulatory agencies, food manufacturers and processors, as well as quality assurance organizations, to detect zeranol in various samples to satisfy customer concerns about food safety.
The unique features of the kit are:
- High recovery (>80%),rapid(10-20minutes), cost-effective extraction methods.
- Detection Limitis 0.32ng/g
- A quick ELISA assay (less than 20min regardless of number of samples).
- High reproducibility.
Procedure Overview
The method is based on a competitive colorimetric ELISA assay. The toxin of interest has been coated in the plate wells. During the analysis, sample is added along with the primary antibody specific for the target toxin and enzyme-conjugate. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the toxin attached to the well. The enzyme-conjugate, will bined with the primary antibody that is complexed to the toxin coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.
Kit Contents, Storage and Shelf Life
REAGEN Zeranol ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused micro wells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C *. The shelf life is 12 months when the kit is properly stored.
| Kit Contents | Amount | Storage |
| Zeranol -Coated Plate | 1x 96-well Plate (8 wells x 12 strips) | 2-8°C |
| Zeranol Standards:
Negative control (white cap tube) 0.1 ng/mL (yellow cap tube) 0.3 ng/mL (orange cap tube) 0.9 ng/mL (pink cap tube) 2.7ng/mL (purple cap tube) 8.1 ng/mL (blue cap tube) 100 ng/mL (spiking, red cap tube) |
0.8 mL 0.8 mL 0.8 mL 0.8 mL 0.8 mL 0.8 mL 0.8 mL |
2-8°C |
| Zeranol Antibody #1 | 7mL | 2-8°C |
| HRP-Conjugated Antibody #2 | 7mL | 2-8°C |
| Dilution Buffer | 7mL | 2-8°C |
| 20X Wash Solution | 28 mL | 2-8°C |
| Stop Buffer | 15 mL | 2-8°C |
| TMB Substrate | 12 mL | 2-8°C |
| 10X PBS | 15 mL | 2-8°C |
| Zeranol Clean Up Reagent（Optional） | 40 g | 2-8°C |
* If you are not planning to use the kit for over 3 months, store Zeranol Antibody #1 and HRP-Conjugated Antibody #2 at -20°C or in a freezer.
Detection Limit Of Quantitation
| Sample Type | Limit of quantitation (ng/g） |
| Meat/fish/shrimp | 0.4 |
| Cereal/feed/seed | 1.0 |
| Milk | 0.5 |
| Urine/Plasma | 0.15 |
Specificity (Cross-Reactivity)
| Analytes | Cross-Reactivity (%) |
| Zeranol | 100.0 |
| Zearalanone | 79 |
| α-Zearalenol | 70 |
| β-Zearalanol | 28 |
| zearalenone | 89 |
| β-Zearalanol | 67 |
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