Doramectin ELISA Test Kit

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Doramectin ELISA Test Kit

$999.00

Doramectin ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of doramectin in meat,fish,shrimp,serum and milk.

Availability: 100 in stock (can be backordered)

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Product Description

REAGEN  Doramectin ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of doramectin in meat,fish,shrimp,serum and milk.

The unique features of the kit are:

  • The extraction of doramectinfrom milk can be finished with a high recovery rate of 75 – 125%.
  • High reproducibility.LOD is 2 ppb.
  • A quick ELISA assay (less than 5hours regardless of number of samples).

Procedure Overview

The method is based on a competitive colorimetric ELISA assay. The drug of interest has been coated in the plate wells. During the analysis, sample is added along with the primary antibody specific for the target drug. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the drug attached to the well. The secondary antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the drug coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.

Kit Contents, Storage and Shelf Life

REAGEN   Doramectin ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused micro-wells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C *. The shelf life is 12 months when the kit is properly stored.

Kit Contents Amount Storage
Doramectin-coated Microtiter Plate 1 x 96-well plate (8 wells x 12 strips) 2-8°C
Doramectin Standards:

Negative control

0.4 ng/mL

0.8 ng/mL

1.6 ng/mL

3.2 ng/mL

6.4 ng/mL

100 ng/mL (spiking, optional)

 

1.0mL

1.0mL

1.0mL

1.0mL

1.0mL

1.0mL

1.0mL

2-8°C *
Doramectin Antibody #1 6 ml 2-8°C
 HRP-Conjugated Ab #2 11 ml
20X Wash Solution 30mL
Stop Buffer 11 mL
TMB Substrate 11 mL
10X Sample Extraction Buffer 15mL

* If you are not planning to use the kit for over 1 month, store DORAMECTIN Standard , Antibody #1,HRP-Conjugated at -20°C preferably or in a freezer.

Limit of quantitation

Sample Type Limit of quantitation (ng/g)
Meat/Fish/Shrimp 2.0
Milk/Serum 2.0

Specificity (Cross-Reactivity)

Molecules Cross-Reactivity (%)
Doramectin 100%
Abamectin 200%
Ivermectin 80%

Brand

REAGEN

Model

RND99069

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IMG_20230823_112650Doramectin ELISA Test Kit
$999.00

Availability: 100 in stock (can be backordered)