Product Description
REAGENÂ Â Furazolidone (AOZ) ELISA Test Kit provides a competitive enzyme mmunoassay for the quantitative analysis of furazolidone in fish, shrimp, meat (chickenă pork and hepar), eggs, honey.
The unique features of the kit are:
- Rapid (4 hours), high recovery (75 – 105%), and cost-effective extraction methods for various samples.
- High sensitivity (0.02ng/g or ppb) and low detection limit (0.04 ng/g or ppb) for various samples.
- A quick ELISA assay (less than 1hours regardless of number of samples).
- High reproducibility.
Procedure Overview
The method is based on a competitive colorimetric ELISA assay. The drug of interest has been coated in the plate wells. During the analysis, sample and the AOZ antibody are added along with the secondary antibody, tagged with a peroxidase enzyme. If the AOZ residue is present in the sample, it will compete for the AOZ antibody, thereby preventing the antibody from binding to the AOZ-OVA attached to the well. The resulting color intensity, after addition of the HRP substrate (TMB), has an inverse relationship with the AOZ residue concentration in the sample.
Kit Contents, Storage and Shelf Life
REAGEN  Furazolidone (AOZ) ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C*. The shelf life is 12 months when the kit is properly stored.
| Kit Contents | Amount | Storage |
| AOZ-coated Microtiter Plate | 96-well plate (8 wells x 12 strips) | 2-8°C |
| AOZ Standards:
Negative control (white cap tube) 0.02Â ng/mL (yellow cap tube) 0.06Â ng/mL (orange cap tube) 0.18Â ng/mL (pink cap tube) 0.54Â ng/mL (purple cap tube) 1.62ng/mL (blue cap tube) 10 ng/mL (spiking, optional, red cap tube) |
1.0Â mL 1.0Â mL 1.0Â mL 1.0Â mL 1.0Â mL 1.0Â mL 1.0Â mL |
2-8°C |
| Antibody #1 | 6 mL | 2-8°C |
| HRP-Conjugated Antibody #2 | 6 mL | 2-8°C |
| 10X Sample Extraction Buffer | 25 mL | 2-8°C |
| 20X Wash Solution | 30 mL | 2-8°C |
| Stop Buffer | 12 mL | 2-8°C |
| TMB Substrate | 12 mL | 2-8°C |
| 50 mM 2-Nitrobenzaldehyde | 2.0 mL | 2-8°C |
Sensitivity (Detection Limit)
| Sample Type | Detection Limit (ng/g or ppb) |
| Shrimp/Fish/Meat/Hepar | 0.04 |
| Egg/Egg Powder | 0.04 |
| Honey | 0.04 |
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