Product Description
REAGEN Sulfamethazine ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Sulfamethazine in eggs, fish, shrimp, meat (beef, chicken and pork), honey, milk, milk powder.
The unique features of the kit are:
- Rapid extraction method for various samples with high recovery (80-105%).
- High sensitivity (0.2ppb).
- High reproducibility.
- A quick ELISA assay (less than 1hours regardless of number of samples).
Procedure Overview
The method is based on a competitive colorimetric ELISA assay. The drug of interest has been coated in the plate wells. During the analysis, sample and the Sulfamethazine antibody are added along with secondary antibody, tagged with a peroxidase enzyme. If the drug residue is present in the sample, it will compete for the Sulfamethazine antibody, thereby preventing the antibody from binding to the drug attached to the well. The resulting color intensity, after addition of the HRP substrate (TMB), has an inverse relationship with the drug residue concentration in the sample.
Kit Contents, Storage and Shelf Life
REAGEN Sulfamethazine ELISA Test Kit has the capacity for 96 determinations of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C *. The shelf life is 12 months when the kit is properly stored.
| Kit Contents | Amount | Storage |
| Sulfamethazine Plate | 1×96-well Plate(8wellsx12 strips) | 2-8°C |
| Sulfamethazine Standards:
Negative control (white cap tube) 0.2ng/mL (yellow cap tube) 0.6 ng/mL (orange cap tube) 1.8 ng/mL (pink cap tube) 5.4ng/mL (purple cap tube) 16.2 ng/mL (blue cap tube) 100 ng/mL(spiking, optional, red cap tube) |
1.0 mL 1.0 mL 1.0 mL 1.0 mL 1.0 mL 1.0 mL 1.0 mL |
2-8°C
2-8°C
|
| Sulfamethazine Antibody #1 | 6 mL | 2-8°C |
| HRP Conjugate | 6 mL | |
| 20X Wash Solution | 30 mL | |
| Stop Buffer | 12 mL | |
| TMB Substrate | 12 mL | |
| 10X Sample Extraction Buffer | 30 mL | |
| 5X Sample Dilution Buffer | 25 mL |
* If you are not planning to use the kit for over 1 month, store Sulfamethazine Antibody, HRP Conjugate at -20°C or in a freezer.
Sensitivity (Detection Limit)
| Sample Type | Detection Limit (ng/g or ppb) |
| Egg | 0.2 |
| Fish/Shrimp/Meat/Liver/Kidney | 0.2/2 |
| Honey | 2 |
| Milk | 0.2 |
| Milk Powder | 0.4 |
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