Product Description
REAGEN Testosterone ELISA Test Kit provides a competitive enzyme immunoassay for the quantitative analysis of Testosterone in milk, fish, shrimp, meat ,plasma and urine.
The unique features of the kit are:
- Rapid (10-40min), high recovery (>80%), and cost-effective extraction methods .
- High sensitivity (0.1 ng/g or ppb) and low detection limit (0.1ng/g or ppb) .
- A quick ELISA assay (less than 5hours regardless of number of samples).
- High reproducibility.
Procedure Overview
The method is based on a competitive colorimetric ELISA assay. The Testosterone has been coated in the plate wells. During the analysis, sample is added along with the HRP-Conjugated Antibody #2 and Testosterone Antibody #1 . If the Testosterone residue is present in the sample, it will compete for the Testosterone antibody #1, thereby preventing the Testosterone from binding to the antibody attached to the well. The resulting color intensity, after addition of the HRP substrate (TMB), has an inverse relationship with the Testosterone residue concentration in the sample.
Kit Contents, Storage and Shelf Life
REAGEN Testosterone ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C*. The shelf life is 12 months when the kit is properly stored.
| Kit Contents | Amount | Storage |
| Testosterone-coated Microtiter Plate | 1 x 96-well plate (8 wells x 12 strips) | 2-8°C |
| Testosterone Standards:
Negative control (white cap tube) 0.1ng/mL (yellow cap tube) 0.3 ng/mL (orange cap tube) 0.9 ng/mL (pink cap tube) 2.7 ng/mL (purple cap tube) 8.1 ng/mL (blue cap tube) 100 ng/mL (spiking, optional, red cap tube) |
1.0 mL 1.0 mL 1.0 mL 1.0 mL 1.0mL 1.0 mL 1.0 mL |
2-8°C |
| Testosterone Antibody #1 | 6 mL | 2-8°C * |
| HRP-Conjugated Antibody #2 | 6 mL | 2-8°C * |
| 5X Milk Sample Dilution | 25 mL | 2-8°C |
| 10X Sample Extraction Buffer | 30 mL | 2-8°C |
| 20X Wash Solution | 30 mL | 2-8°C |
| Stop Buffer | 12 mL | 2-8°C |
| TMB Substrate | 12 mL | 2-8°C |
| Clean up mix（optional） | 30ml | 2-8°C |
* If you are not planning to use the kit for over 3 months, store Antibody #1 and HRP-
Conjugated Antibody #2 at -20°C or in a freezer.
Sensitivity (Detection Limit)
| Sample Type | Detection Limit (ng/g or ppb) |
| Milk | 1.0 |
| Shrimp/Fish/Meat | 0.1 |
| Plasma/Serum | 0.05 |
| Urine | 0.5 |
Specificity (Cross-Reactivity)
| Analytes | Cross-Reactivity (%) |
| Testosterone | 100 |
| Methyltestosterone | 0.9 |
| Cortisol | 0.1 |
| Progesterone | 0.4 |
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