Product Description
REAGENĀ Ā Zilpaterol ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Zilpaterol in feed, tissue, urine, serum, plasma, milk.
The unique features of the kit are:
- Rapid (10 – 30 minutes), and organic reagent-free extraction method for various samples with high recovery (75- 120%)
- High sensitivity (0.05ng/g or ppb)
- A quick ELISA assay (less than 2 hours regardless of number of samples)
- High reproducibility
ProcedureĀ Overview
Zilpaterol ELISA test kit is based on the principle of competitive enzyme-linked reaction. The antibody containing zilpaterol has been coated on the microplate. For drug analysis, the sample and specific antibody are added to the wells of the plate together. If the sample contains a drug, it will compete for the antibody and inhibit the binding of the antigen to the drug antibody coated on the plate. Add enzyme-labeled secondary antibody to form a coating antibody-antigen-enzyme-labeled complex. After adding the substrate, the color intensity of the product is inversely proportional to the concentration of the drug in the sample.
Kit Contents, StorageĀ and Shelf Life
REAGENĀ Ā Zilpaterol ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8Ā°C Ā The shelf life is 12 months when the kit is properly stored.
| Kit Contents | Amount | Storage |
| Zilpaterol-coated Microtiter Plate
|
1 x 96-well plate (8 wells x 12 strips) | |
| Zilpaterol Standards:
Negative control 0.05Ā ng/mL 0.15Ā ng/mL 0.45Ā ng/mL 1.35Ā ng/mL 4.05Ā ng/mL 10Ā ng/mL(spiking, optional, red cap tube) |
1Ā mL 1Ā mL 1Ā mL 1Ā mL 1Ā mL 1Ā mL 1mL
|
2-8Ā°C
|
| ZilpaterolĀ Antibody #1 | 6mL |
2-8Ā°C
|
| HRP-Conjugated Antibody#2 | 6ml | |
| 20ĆWash Solution | 30mL | |
| Stop Buffer | 12mL | |
| TMB Substrate | 12mL | |
| 10ĆSample Dilution Buffer | 25mL | |
| SampleĀ Extraction Buffer Aļ¼optionalļ¼ | 5ml | |
| SampleĀ Extraction Buffer Bļ¼optionalļ¼ | 5ml |
* Ā If you are not planning to use the kit for over 3 months, store Zilpaterol Antibody #1Ā and HRP-ConjugatedĀ Ā Antibody#2Ā working buffer at -20Ā°C or in a freezer.
Sensitivity (Detection Limit)
| Sample Type | Detection Ā Limit ( ppb) |
| Feed | 1 |
| TissueĀ (muscle/liver) | 0.2 |
| Milk | 0.22 |
| Urine/Serum/Plasma | 0.05 |
Specificity (Cross-Reactivity)
| Analytes | Cross-Reactivity (%) |
| Zilpaterol | 100 |
| Mabuterol | 5.8 |
| Mapenterol | 4.2 |
| Tolubuterol | 2.8 |
| Terbutaline | 2.3 |
| Cimbuterol | 1.7 |
| Salbutamol | 1.0 |
| Cimaterol | 0.6 |
| Pirbuterol | 0.4 |
| Isoprenaline | 0.2 |
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