Zilpaterol ELISA Test Kit

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Zilpaterol ELISA Test Kit

$999.00

Zilpaterol ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Zilpaterol in feed, tissue, urine, serum, plasma, milk.

Availability: 100 in stock (can be backordered)

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Product Description

REAGEN  Zilpaterol ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Zilpaterol in feed, tissue, urine, serum, plasma, milk.

The unique features of the kit are:

  1. Rapid (10 – 30 minutes), and organic reagent-free extraction method for various samples with high recovery (75- 120%)
  2. High sensitivity (0.05ng/g or ppb)
  3. A quick ELISA assay (less than 2 hours regardless of number of samples)
  4. High reproducibility

Procedure Overview

Zilpaterol ELISA test kit is based on the principle of competitive enzyme-linked reaction. The antibody containing zilpaterol has been coated on the microplate. For drug analysis, the sample and specific antibody are added to the wells of the plate together. If the sample contains a drug, it will compete for the antibody and inhibit the binding of the antigen to the drug antibody coated on the plate. Add enzyme-labeled secondary antibody to form a coating antibody-antigen-enzyme-labeled complex. After adding the substrate, the color intensity of the product is inversely proportional to the concentration of the drug in the sample.

Kit Contents, Storage and Shelf Life

REAGEN  Zilpaterol ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C  The shelf life is 12 months when the kit is properly stored.

Kit Contents Amount Storage
Zilpaterol-coated Microtiter Plate

 

1 x 96-well plate (8 wells x 12 strips)
Zilpaterol Standards:

Negative control

0.05 ng/mL

0.15 ng/mL

0.45 ng/mL

1.35 ng/mL

4.05 ng/mL

10 ng/mL(spiking, optional, red cap tube)

 

1 mL

1 mL

1 mL

1 mL

1 mL

1 mL

1mL

 

 

2-8°C

 

 

 

 

 

Zilpaterol Antibody #1 6mL  

 

 

 

 

2-8°C

 

HRP-Conjugated Antibody#2 6ml
20×Wash Solution 30mL
Stop Buffer 12mL
TMB Substrate 12mL
10×Sample Dilution Buffer 25mL
Sample Extraction Buffer A(optional) 5ml
Sample Extraction Buffer B(optional) 5ml

*  If you are not planning to use the kit for over 3 months, store Zilpaterol Antibody #1 and HRP-Conjugated  Antibody#2 working buffer at -20°C or in a freezer.

Sensitivity (Detection Limit)

Sample Type Detection  Limit ( ppb)
Feed 1
Tissue (muscle/liver) 0.2
Milk 0.22
Urine/Serum/Plasma 0.05

Specificity (Cross-Reactivity)

Analytes Cross-Reactivity (%)
Zilpaterol 100
Mabuterol 5.8
Mapenterol 4.2
Tolubuterol 2.8
Terbutaline 2.3
Cimbuterol 1.7
Salbutamol 1.0
Cimaterol 0.6
Pirbuterol 0.4
Isoprenaline 0.2
Brand

REAGEN

Model

RND99040

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Product ImageZilpaterol ELISA Test Kit
$999.00

Availability: 100 in stock (can be backordered)