Product Description
REAGEN Danofloxacin ELISA Test Kit provides a competitive enzyme mmunoassay for the quantitative analysis of Danofloxacin in honey,fish,shrimp,meat,tissue,milk,serum and urine.
The unique features of the kit
- Rapid, high recovery (75 – 95%), and cost-effective extraction methods for various samples.
- High sensitivity (0.1 ng/g or ppb) and low detection limit.
- High reproducibility.
- A quick ELISA assay (less than 1.5 hour regardless of number of samples).
Procedure Overview
The method is based on a competitive colorimetric ELISA assay. The drug of interest has been coated in the plate wells. During the analysis, sample and HRP-Conjugated Antibody #2 are added along with the primary antibody specific for the target drug. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the drug attached to the well. At the same time, the second Antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the drug coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.
Kit Contents, Storage and Shelf Life
REAGEN Danofloxacin ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C . The shelf life is 12 months when the kit is properly stored.
| Kit Contents | Amount | Storage |
| Danofloxacin Coated Plate | 1 x 96-well Plate (8 wells x 12 strips) | 2-8°C |
| Danofloxacin Standards:
Negative control (white cap tube) 0.1 ng/mL (yellow cap tube) 0.3 ng/mL (orange cap tube) 0.9 ng/mL (pink cap tube) 2.7 ng/mL (purple cap tube) 8.1 ng/mL (blue cap tube) 100ng/mL (spiking, optional, red cap tube) |
1.0 mL 1.0 mL 1.0 mL 1.0 mL 1.0 mL 1.0 mL 1.0 mL |
2-8°C |
| Danofloxacin Antibody 1# | 6.0 mL | 2-8°C |
| HRP Conjugate Antibody 2# | 6.0 mL | 2-8°C |
| 10 X Sample Dilution Buffer | 25mL | 2-8°C |
| 20X Wash Solution | 30 mL | 2-8°C |
| Stop Buffer | 12 mL | 2-8°C |
| TMB Substrate | 12 mL | 2-8°C |
| 30 X Sample Extraction Buffer | 15 mL | 2-8°C |
*If you are not planning to use the kit for over 1 month, store Danofloxacin Standards, Danofloxacin Antibody and HRP-Conjugate at -20°C or in a freezer.
Sensitivity (Detection Limit)
| Sample Type | Detection Limit (ng/g or ppb) |
| Honey | 1 |
| Meat/Liver/Kidney/Fish/Shrimp/Egg | 1/0.2 |
| Milk | 0.5 |
| Serum /Urine | 0.5 |
Specificity (Cross-Reactivity)
| Analytes | Cross-Reactivity (%) |
| Danofloxacin | 100 |
| Enrofloxacin | 45 |
| Sarafloxacin | 14 |
| Ofloxacin | 8 |
| Benofloxacin | 3 |
| Oxolin acid | 4 |
| Nalidixic acid | 2 |
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