Product Description
REAGEN Ribavirin ELISA Test Kit enables government agencies, food manufacturers and quality assurance organizations to detect ribavirin in meat,feed to satisfy customer concerns about food safety.
The unique features of the kit are:
- The extraction of ribavirinfrom samples can be finished in 30 minutes with a high recovery rate of 80 – 115%.
- A quick ELISA assay (less than 3hours regardless of number of samples).
- High reproducibility.
Procedure Overview
The method is based on a competitive colorimetric ELISA assay. The drug of interest has been coated in the plate wells. During the analysis, sample is added along with the primary antibody specific for the target drug. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the drug attached to the well. The secondary antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the drug coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.
Kit Contents, Storage and Shelf Life
REAGEN Ribavirin ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C *. The shelf life is 12 months when the kit is properly stored.
Kit Contents | Amount | Storage |
Ribavirin -Coated Plate | 1×96-well Plate (8wells x12strips) | 2-8°C |
Ribavirin Standards:
Negative control 0.1ppm 0.3ppm 0.9ppm 2.7ppm 8.1ppm |
1.0 mL 1.0 mL 1.0 mL 1.0 mL 1.0 mL 1.0 mL |
2-8°C |
HRP-Ab2# Conjugate(red cap tube) | 12mL | 2-8℃ |
Ribavirin Ab 1#(green cap tube) | 7mL | |
2X Sample Diluent (blue cap tube) | 50 mL | |
20X Wash Solution (white cap tube) | 40 mL | |
Stop Buffer (yellow cap tube) | 7 mL | |
Substrate A (brown cap tube) | 7 mL | |
Substrate B (black cap tube) | 7 mL |
Sensitivity (Detection Limit)
Sample Type | Detection Limit (ppm) |
Milk | 2 |
Tissue | 0.15 |
Specificity (Cross-Reactivity)
Analytes | Cross-Reactivity (%) |
Ribavirin | 100.0 |
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