Histamine ELISA Test Kit

IMG_20230823_112650

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IMG_20230823_112650

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Algal Toxins, Food Safety

Histamine ELISA Test Kit

Histamine Enzymatic Assay Kit is a colorimetric enzymatic assay for the determination of histamine in fish meal/seafood, milk,wine.

Product Description

REAGEN Histamine Enzymatic Assay Kit is a colorimetric enzymatic assay for the determination of histamine in fish meal/seafood, milk,wine.

The unique features of the kit are:

Rapid, cost-effective aqueous extraction method with high recovery.
New methanol-free extraction protocol
High sensitivity and low detection limit (3ppm for fresh fish/seafood).
High reproducibility.
A very rapid (10 minutes) and robust enzyme-based assay which does not require

Procedure Overview

The REAGEN Histamine Enzymatic Assay Kit measures the chemical reduction of a dye molecule using a histamine-specific enzyme. The color change caused by reduction of the dye (measured by the absorbance at 450 nm) is related to the amount of histamine present in the sample.

Kit Contents, Storage and Shelf Life

Kit Contents	Amount	Storage
Histamine-Microtiter Plate	1x 96-well Plate (8wells x 12 strips)	2-8°C
Histamine standards : Negative control (white cap tube) 1.0 ppm (yellow cap tube) 2.0 ppm (orange cap tube) 4.0 ppm (pink cap tube) 8.0 ppm (purple cap tube) 12.0 ppm (blue cap tube) 1,000 ppm (spiking, optional, red cap tube)	0.8 mL 0.8 mL 0.8 mL 0.8 mL 0.8 mL 0.8 mL 0.8 mL	2-8°C
Master Mix **	12 mL	2-8°C
Enzyme Mix	12mL	2-8°C
100X Enrichment Solution	2X25mL	2-8°C
6X Sample Extraction	25mL	2-8°C

Sensitivity (Detection Limit)

Sample Type	Detection Limit (mg/kg or ppm)
Seafood(Tuna, Mahi)	3
Fish Meal	10
Milk	40
Wine	40

Specificity (Cross-Reactivity)

Analytes	Cross-Reactivity (%)
Histamine	100

Brand	REAGEN
Model	RNA97010

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