Histamine ELISA Test Kit

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Histamine ELISA Test Kit

Histamine Enzymatic Assay KitĀ is a colorimetric enzymatic assay for the determination of histamine in fish meal/seafood, milk,wine.

Product Description

REAGENĀ Ā Histamine Enzymatic Assay KitĀ is a colorimetric enzymatic assay for the determination of histamine in fish meal/seafood, milk,wine.

The unique features of the kit are:

  • Rapid, cost-effective aqueous extraction method with high recovery.
  • New methanol-free extraction protocol
  • High sensitivity and low detection limit (3ppm for fresh fish/seafood).
  • High reproducibility.
  • A very rapid (10 minutes) and robust enzyme-based assay which does not require

ProcedureĀ Overview

TheĀ REAGENĀ Ā Histamine Enzymatic AssayĀ Kit measures the chemical reduction of a dye molecule using a histamine-specific enzyme. The color change caused by reduction of the dye (measured by the absorbance at 450 nm) is related to the amount of histamine present in the sample.

Kit Contents, StorageĀ and Shelf Life

Kit Contents Amount Storage
Histamine-Microtiter Plate 1x 96-well Plate (8wells x 12 strips) 2-8Ā°C
Histamine standards :

Negative controlĀ (white cap tube)

1.0Ā ppm (yellow cap tube)

2.0Ā ppm (orange cap tube)

4.0Ā ppm (pink cap tube)

8.0Ā ppm (purple cap tube)

12.0Ā ppm (blue cap tube)

1,000 ppm (spiking, optional, red cap tube)

0.8Ā mL

0.8Ā mL

0.8Ā mL

0.8Ā mL

0.8Ā mL

0.8Ā mL

0.8Ā mL

2-8Ā°C
Master Mix ** 12Ā mL 2-8Ā°C
Enzyme Mix 12mL 2-8Ā°C
100X Enrichment Solution 2X25mL 2-8Ā°C
6X Sample Extraction 25mL 2-8Ā°C

Sensitivity (Detection Limit)

Sample Type Detection Limit (mg/kg or ppm)
Seafood(Tuna, Mahi) 3
Fish Meal 10
Milk 40
Wine 40

Specificity (Cross-Reactivity)

Analytes Cross-Reactivity (%)
Histamine 100
Brand

REAGEN

Model

RNA97010

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