Product Description
REAGEN Okadaic Acid (DSP) ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Okadaic Acid (DSP) in mussels and water samples.
The unique features of the kit are:
- High recovery (80-105%), rapid (10-30 minutes), and cost-effective extraction methods
- High sensitivity (0.3ng/g or ppb) and low detection limit (30 ppb for mussel samples and 6 ppb for water samples).
- High reproducibility.
- A quick ELISA assay(less than 1.5 hours regardless of number of samples).
Procedure Overview
The method is based on a competitive colorimetric ELISA assay. The second antibody against Anti-Okadaic Acid Antibody was coated in the plate wells. During the analysis, sample is added along with the okadaic acid-horseradish peroxidase conjugate (Okadaic Acid-HRP Conjugate) and Anti- Okadaic Acid Antibody. If the okadaic acid residue is present in the sample, it will compete with Okadaic Acid-HRP Conjugate for the Anti-Okadaic Acid Antibody, thereby preventing the Okadaic Acid-HRP Conjugate from binding to the Anti-Okadaic Acid Antibody. The Anti-Okadaic Acid Antibody is then bound by the second antibody immobilized on the plate. After a washing step and addition of the TMB substrate solution, a color signal is produced. The color reaction is stopped after a specified time and the color is evaluated using an ELISA reader. The resulting color intensity has an inverse relationship with the okadaic acid residue concentration in the sample.
Kit Contents, Storage and Shelf Life
REAGEN Okadaic Acid (DSP) ELISA Test Kit has the Okadaic Acid (DSP) capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8℃ . The shelf life is 12 months when the kit is properly stored.
| Kit Contents | Amount | Storage |
| DSP Second Antibody-coated Plate | 1 x 96-well Plate(8 wells x 12 strips) | 2-8℃ |
| Okadaic Acid Standards:
Negative control (white cap tube) 0.3ng/mL (yellow cap tube) 0.9ng/mL (orange cap tube) 2.7ng/mL (pink cap tube) 5.4ng/mL (purple cap tube) 16.2ng/mL (blue cap tube) |
0.8mL 0.8 mL 0.8 mL 0.8mL 0.8 mL 0.8mL |
-20℃ |
| Anti-Okadaic Acid Antibody | 6 ml | 2-8℃ |
| Okadaic Acid-HRP Conjugate | 6 mL | |
| 20X Wash Solution | 30 mL | |
| Stop Buffer | 14mL | |
| TMB Substrate | 12mL | |
| 10X Sample Extraction Buffer | 25 mL |
* If you are not planning to use the kit for over 1 months, store Okadaic Acid-HRP Conjugateand Anti- Okadaic Acid Antibody at -20℃ or in a freezer.
Sensitivity (Detection Limit)
| Sample Type | Detection Limit (ng/g or ppb) |
| Mussels | 30 |
| Water | 6 |
Specificity (Cross-Reactivity)
| Analytes | Cross-Reactivity (%) |
| Okadaic Acid | 100.0 |
| DTX1 | 40 |
| DTX2 | 46 |
| Neosaxitoxin | < 0.01 |
| Saxitoxin | < 0.01 |
| YTX | < 0.01 |
Reviews
There are no reviews yet.