KOD DNA Polymerase

KOD DNA Polymerase

$999.00

KOD DNA polymerase was isolated and purified from Thermococcus Kodakaraensis DNA polymerase gene by induced expression in Escherichia coli. Due to its strong 3 ‘→ 5’ exonuclide activity, the Polymerase has a higher amplification fidelity than Pfu DNA Polymerase, which is about 50 times that of Taq. At the same time, the polymerase has a faster synthesis speed, polymerization rate is about 5 times that of common Pfu DNA Polymerase. Taq DNA Polymerase, which is twice the size of TAQ DNA, reaches 100-138bp/s, and can be polymerase with high yield in a short time. It is especially suitable for high-fidelity amplification of PCR products less than 6kb, and the resulting flat-ended DNA can be used in molecular biology experiments such as gene cloning, expression and mutation analysis.

Availability: 100 in stock (can be backordered)

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Product overview

KOD DNA polymerase was isolated and purified from Thermococcus Kodakaraensis DNA polymerase gene by induced expression in Escherichia coli. Due to its strong 3 ‘→ 5’ exonuclide activity, the Polymerase has a higher amplification fidelity than Pfu DNA Polymerase, which is about 50 times that of Taq. At the same time, the polymerase has a faster synthesis speed, polymerization rate is about 5 times that of common Pfu DNA Polymerase. Taq DNA Polymerase, which is twice the size of TAQ DNA, reaches 100-138bp/s, and can be polymerase with high yield in a short time. It is especially suitable for high-fidelity amplification of PCR products less than 6kb, and the resulting flat-ended DNA can be used in molecular biology experiments such as gene cloning, expression and mutation analysis.

Product characteristics

• Higher fidelity than Pfu DNA polymerase, suitable for cloning

The yield expansion rate is 2 times faster than Taq DNA polymerase and 5 times faster than Pfu DNA polymerase

Sequence nucleotide polymerization is 10-15 times higher than Pfu and Tli DNA polymerase

• Amplifies plasmid and lambda DNA template up to 6 kbp

• Amplified genomic DNA template up to 2 kbp

• Amplification products without truncation

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• Rapid amplification of high-fidelity DNA fragments

• Rich in GC and complex template amplification

• Flat end cloning

• site-specific mutation

Quality Control

The purity was greater than 90% as determined by SDS-PAGE, and no exogenous nuclease activity was detected.

Mouse or human genomic DNA can be effectively amplified without host residual DNA.

PCR Protocol

1.Operation steps for a 50µL reaction system:

         Components            Volume          Final Concentration
         KOD DNA Polymerase 0.25-0.5 µL 1.25-2.5units
5×KOD Reaction Buffer 10 µL 1X
dNTP Mix(2.5mM each) 4 µL 0.2-0.4 mM
Primer 1 (50µM) 0.5-1 µL 0.5-1 µM
Primer 2 (50µM) 0.5-1 µL 0.5-1 µM
Template DNA 1 µL As required
PCR Water Up to 50µL N/A
Brand

REAGEN

Model

RNR98004

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Product PictureKOD DNA Polymerase
$999.00

Availability: 100 in stock (can be backordered)