KOD DNA polymerase was isolated and purified from Thermococcus Kodakaraensis DNA polymerase gene by induced expression in Escherichia coli. Due to its strong 3 ‘→ 5’ exonuclide activity, the Polymerase has a higher amplification fidelity than Pfu DNA Polymerase, which is about 50 times that of Taq. At the same time, the polymerase has a faster synthesis speed, polymerization rate is about 5 times that of common Pfu DNA Polymerase. Taq DNA Polymerase, which is twice the size of TAQ DNA, reaches 100-138bp/s, and can be polymerase with high yield in a short time. It is especially suitable for high-fidelity amplification of PCR products less than 6kb, and the resulting flat-ended DNA can be used in molecular biology experiments such as gene cloning, expression and mutation analysis.
• Higher fidelity than Pfu DNA polymerase, suitable for cloning
The yield expansion rate is 2 times faster than Taq DNA polymerase and 5 times faster than Pfu DNA polymerase
Sequence nucleotide polymerization is 10-15 times higher than Pfu and Tli DNA polymerase
• Amplifies plasmid and lambda DNA template up to 6 kbp
• Amplified genomic DNA template up to 2 kbp
• Amplification products without truncation
• Rapid amplification of high-fidelity DNA fragments
• Rich in GC and complex template amplification
• Flat end cloning
• site-specific mutation
The purity was greater than 90% as determined by SDS-PAGE, and no exogenous nuclease activity was detected.
Mouse or human genomic DNA can be effectively amplified without host residual DNA.
1.Operation steps for a 50µL reaction system:
|KOD DNA Polymerase
|5×KOD Reaction Buffer
|dNTP Mix(2.5mM each)
|Primer 1 (50µM)
|Primer 2 (50µM)
|Up to 50µL