Taq DNA Polymerase is one of the most commonly used DNA polymerases.
Taq DNA Polymerase is a highly thermally stable DNA polymerase derived from Thermus aquaticus with a half-life greater than 40 minutes when incubated at 95℃. The molecular weight of Taq enzyme is 94 kDa. The Taq enzyme catalyzes the polymerization of deoxynucleotides in the 5′ to 3′ direction dependent on the DNA template. The Taq enzyme has no 3′ to 5′ exonuclease activity and has very low 5′ to 3′ exonuclease activity. Since the Taq enzyme has no 3′ to 5′ exonuclease activity, it acts as A nucleotide transferase during DNA polymerization, resulting in a 3′-dA overhangs on the 3′ end of the PCR product, i.e., a 3′ sticky end with an A
There is no contamination of exogenous nuclease and fine DNA
High amplification efficiency
• Routine PCR signing
• Small fragment target gene cloning
• PCR-based DNA fingerprinting analysis
• Other primer extension reactions, such as sequencing and labeling