Product Description
REAGEN Nicarbazin ELISA Test Kit enables government agencies, food manufacturers and quality assurance organizations to detect nicarbazin in Feed,Chicken,Pork,Egg and Beef to satisfy customer concerns about food safety.
The unique features of the kit are:
- The extraction of nicarbazin from samples can be finished in 10 – 30 minutes with a high recovery rate of 75- 95%.
- A quick ELISA assay (less than 1.5 hours regardless of number of samples).
- High reproducibility.
Procedure Overview
The method is based on a competitive colorimetric ELISA assay. The nicarbazin antibody has been coated in the plate wells. During the analysis, sample is added along with the Nicarbazin-horseradish peroxidase conjugate. If the nicarbazin residue is present in the sample, it will compete for the nicarbazin antibody, thereby preventing the nicarbazin-HRP from binding to the antibody attached to the well. The resulting color intensity, after addition of the HRP substrate (TMB), has an inverse relationship with the nicarbazin residue concentration in the sample.
Kit Contents, Storage and Shelf Life
REAGEN Nicarbazin ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C *. The shelf life is 12 months when the kit is properly stored.
| Kit Contents | Amount | Storage |
| Nicarbazin Ab-Coated Plate | 1×96-well Plate (8wells x12strips) | 2-8°C |
| Nicarbazin Standards:
Negative control (white cap tube) 1 ng/mL (yellow cap tube) 2 ng/mL (orange cap tube) 4 ng/mL (pink cap tube) 8 ng/mL (purple cap tube) 16 ng/mL (blue cap tube) 16000ng/mL(spiking, optional, red cap tube) |
2.0 mL 2.0 mL 2.0 mL 2.0 mL 2.0 mL 2.0 mL 2.0 mL
|
2-8°C |
| Nicarbazin-HRP Conjugate | 6 mL | 2-8°C * |
| 10X Sample Diluent ** | 15 mL | 2-8°C |
| 20X Wash Solution ** | 30 mL | 2-8°C |
| Stop Buffer ** | 14 mL | 2-8°C |
| TMB Substrate ** | 12 mL | 2-8°C |
| 10XPBS | 25 ml | 2-8°C |
* If you are not planning to use the kit for over 3 months, store Nicarbazin-HRP Conjugate at -20°C or in a freezer.
Sensitivity (Detection Limit)
| Sample Type | Detection Limit (ng/g or ppb) |
| Feed | 250 |
| Chicken/Pork/Beef | 50 |
| Egg | 50 |
Specificity (Cross-Reactivity)
| Analytes | Cross-Reactivity (%) |
| Nicarbazin | 100.0 |
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