Tilmicosin ELISA Test Kit

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Tilmicosin ELISA Test Kit

$999.00

Tilmicosin ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of tilmicosin in bran mash, egg, honey, meat/liver/kidney, milk and urine.

Availability: 100 in stock (can be backordered)

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Product Description

REAGEN  Tilmicosin ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of tilmicosin in bran mash, egg, honey, meat/liver/kidney, milk and urine.

The unique features of the kit are:

  • Rapid (10-40 minutes), and organic reagent-free extraction method for various samples withhigh recovery (>80%).
  • High sensitivity (25ng/g or ppb) .
  • High reproducibility.
  • A quick ELISA assay (less than 2 hours regardless of number of samples).

Procedure Overview

The method is based on a competitive colorimetric ELISA assay. The drug of interest has been coated in the plate wells. During the analysis, sample is added along with the primary antibody specific for the target drug. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the drug attached to the well. The secondary antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the drug coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.

Kit Contents, Storage and Shelf Life

REAGEN  Tilmicosin ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Most of the kit components should be stored at 2-8°C except for the Tilmicosin Standards, which should be stored at -20°C (4°C for 1 week). The shelf life is 12 months when the kit is properly stored.

Kit Contents Amount Storage
Tilmicosin-coated Microtiter Plate 1 x 96-well plate (8 x 12 strips) 2-8°C
Tilmicosin Standards:

Negative control (white cap tube)

0.25 ng/mL (yellow cap tube)

1 ng/mL (orange cap tube)

2.5 ng/mL (pink cap tube)

5 ng/mL (purple cap tube)

10 ng/mL (blue cap tube)

1000 ng/mL (spiking,  red cap tube)

 

1.0mL

1.0mL

1.0mL

1.0mL

1.0mL

1.0mL

1.0mL

2-8°C
Tilmicosin Antibody #1 12mL 2-8°C *
100X HRP-Conjugated Antibody #2 250mL
Antibody #2 Diluent ** 20mL
20X Wash Solution ** 30mL
TMB Substrate ** 12mL
Stop Buffer ** 14mL
Sample Extraction Buffer 2x25mL
Honey Balance Buffer (Optional) 2x25mL 2-8°C

* If you are not planning to use the kit for over 1 month, store Tilmicosin Antibody #1 and 100X HRP-Conjugated Antibody #2 at -20°C or in a freezer.

Sensitivity (Detection Limit)

Sample Type Detection Limit (ng/g or ppb)
Bran Mash 2.5(RapidMethod);0.625(Organic Extraction Method)
Egg 2.5 (Rapid Method);0.625 (Organic Extraction Method)
Honey 0.5
Milk 2.5
Meat/Liver/Kidney 2.5
Urine 5

Specificity (Cross-Reactivity)

Analytes Cross-Reactivity (%)
Tilmicosin 100
Spyramicin 10
Tetracyclines < 0.01
Gentamicin < 0.01
Ampicillin < 0.01
Florfenicol < 0.01
Brand

REAGEN

Model

RND99064

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Product ImageTilmicosin ELISA Test Kit
$999.00

Availability: 100 in stock (can be backordered)