Product Description
REAGEN Olaquindox ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Olaquindox in tissue and feed.
The unique features of the kit are:
- High recovery (75% – 105%) .
- Sensitivity:(5ppb)
- High reproducibility.
- A quick ELISA assay.
Procedure Overview
The method is based on a competitive colorimetric ELISA assay. The Olaquindox has been coated in the plate wells. During the analysis, sample is added along with the Olaquindox antibody. If the Olaquindox residue is present in the sample, it will compete for the Olaquindox antibody, thereby preventing the antibody attached to the well. The resulting color intensity, after addition of the HRP substrate (TMB), has an inverse relationship with the Olaquindox residue concentration in the sample.
Kit Contents, Storage and Shelf Life
REAGEN Olaquindox ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C*. The shelf life is 12 months when the kit is properly stored.
| Kit Contents | Amount | Storage |
| Olaquindox -Coated Plate | 96-well plate (8 wells x 12 strips) | 2-8°C |
| Olaquindox Standards:
Negative control 0.5ng/mL 1.5ng/mL 4.5 ng/mL 13.5ng/mL 40.5ng/mL |
1.0 mL 1.0 mL 1.0 mL 1.0 mL 1.0 mL 1.0 mL |
2-8°C |
| 1000 ng/mL (spiking, optional) | 1.0 mL | 2-8°C |
| Olaquindox- HRP Conjugate | 11 mL | |
| Olaquindox Antibody | 5.5 mL | |
| 20X Wash Solution | 40 mL | |
| Stop Buffer | 6 mL | |
| Substrate TMB | 12 mL | |
| 2X Sample Extraction Buffer | 50 mL |
Sensitivity (Detection Limit)
| Sample Type | Detection Limit (ng/g or ppb) |
| Tissue | 1.5 |
| Feed | 150 |
Specificity (Cross-Reactivity)
| Analytes | Cross-Reactivity (%) |
| Olaquindox | 100 |
| Carbadox | <0.1 |
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