Thiamphenicol ELISA Test Kit

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Thiamphenicol ELISA Test Kit

$999.00

Thiamphenicol ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Thiamphenicol in feed, fish, eggs, honey, meat (beef, chicken and pork), milk, milk powder, serum, shrimp and urine.

Availability: 100 in stock (can be backordered)

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Product Description

REAGEN  Thiamphenicol ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Thiamphenicol in feed, fish, eggs, honey, meat (beef, chicken and pork), milk, milk powder, serum, shrimp and urine.

The unique features of the kit are:

  • High recovery (70-115%), rapid (10 – 40 minutes), and cost-effective extraction methods.
  • High sensitivity (0.1ng/g or ppb) and low detection limit (0.05 ng/g or ppb) for shrimp, fish and meat samples.
  • High reproducibility.
  • A quick ELISA assay (less than 1hour regardless of number of samples).

Procedure Overview

The method is based on a competitive colorimetric ELISA assay. The drug of interest has been coated in the plate wells. During the analysis, sample and HRP-Conjugated Antibody #2 are added along with the primary antibody specific for the target drug. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the drug attached to the well. At the same time, the second Antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the drug coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.

Kit Contents, Storage and Shelf Life

REAGEN  Thiamphenicol ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C *. The shelf life is 12 months when the kit is properly stored.

Kit Contents Amount Storage
Thiamphenicol Antibody-Coated Plate 1×96-well Plate (8wells x12strips) 2-8°C
Thiamphenicol Standards:

Negative control (white cap tube)

0.1 ng/mL (yellow cap tube)

0.3 ng/mL (orange cap tube)

0.9 ng/mL (pink cap tube)

2.7 ng/mL (purple cap tube)

8.1 ng/mL (blue cap tube)

100ng/mL(spiking, optional, red cap tube)

0.8 mL

0.8 mL

0.8 mL

0.8 mL

0.8 mL

0.8 mL

0.8 mL

2-8°C
Thiamphenicol Antibody 1# 6mL 2-8°C
HRP Conjugate Antibody 2# 6mL 2-8°C
10X Sample Extraction Buffer ** 25mL 2-8°C
Reagent A 18mL 2-8°C
Reagent B 18mL 2-8°C
20X Wash Solution ** 28mL 2-8°C
Stop Buffer ** 12mL 2-8°C
TMB Substrate ** 11mL 2-8°C

Sensitivity (Detection Limit)

Sample Type Detection Limit (ng/g or ppb)
Milk 0.1
Fish/Shrimp/Meat/Egg 0.05

Specificity (Cross-Reactivity)

Analytes Cross-Reactivity (%)
Thiamphenicol 100
Chloramphenicol Glucuronide < 0.5
Chloramphenicol Base < 0.5
Chloramphenicol)CAP < 0.05
Tetracyclines < 0.01
Gentamicin < 0.01
Ampicillin < 0.01
Florfenicol < 0.01
Brand

REAGEN

Model

RND99096

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IMG_20230915_174149Thiamphenicol ELISA Test Kit
$999.00

Availability: 100 in stock (can be backordered)