Product Description
REAGEN MQCA ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of MQCA in egg, tissue, pig liver, serum, urine.
The unique features of the kit are:
- Highrecovery (>80%),rapid(10-40minutes),and cost-effective extraction methods.
- High sensitivity(0.2ng/g or ppb).
- A quick ELISA assay(less than 1 hours regardless of number of samples).
- High reproducibility.
Procedure Overview
The method is based on a competitive colorimetric ELISA assay. The drug of interest has been coated in the plate wells. During the analysis, sample is added along with the primary antibody specific for the target toxin and enzyme-conjugate. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the drug attached to the well. The enzyme-conjugate, will bind with the primary antibody that is complexed to the drug coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.
Kit Contents, Storage and Shelf Life
REAGEN MQCA ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C *. The shelf life is 12 months when the kit is properly stored.
| Kit Contents | Amount | Storage |
| MQCA Coated Plate | 1 x 96-well Plate (8 wells x 12 strips) | 2-8°C |
| MQCA Standards:
Negative control (white cap tube) 0.2 ng/mL (yellow cap tub) 0.6ng/mL (orange cap tube) 1.8ng/mL (pink cap tube) 5.4ng/mL (purple cap tube) 16.2ng/mL (blue cap tube) 100ng/mL(spiking,optional,redcap tube) |
1.0 mL 1.0 mL 1.0 mL 1.0 mL 1.0 mL 1.0 mL 1.0 mL |
2-8°C * |
| MQCA Antibody | 6mL | 2-8°C * |
| 1X HRP Conjugate | 11mL | 2-8°C * |
| 20X Wash Solution | 40mL | 2-8°C |
| Stop Buffer | 6 mL | 2-8°C |
| TMB Substrate Buffer | 12 mL | 2-8°C |
| 2X Sample Dilution Buffer | 50 mL | 2-8°C |
*If you are not planning to use the kit for over 1 month ,MQCA Antibody #1 and 1×HRP-Conjugated at －20℃ or in a freezer is recommended.
Sensitivity (Detection Limit)
| Sample Type | Detection Limit (ng/g or ppb) |
| Egg, Tissue | 0.4 |
| Pig Liver | 0.8 |
| Serum, Urine | 1 |
Specificity (Cross-Reactivity)
| Analytes | Cross-Reactivity (%) |
| MQCA | 100 |
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