Product Description
REAGENĀ Ā Folic acid Test Kit is quantitative analysis of total Folic acid in food ,feed and drug.
The unique features of the kit are:
- rapid, and cost-effective extraction methods,the same as other Vitamin
- The kit provide positive control and serial standards
- High sensitivity (0.05Ī¼g/100g) and low detection limit (0.0125Ī¼g/100g)
- High recoveryļ¼80%-115%ļ¼
- High reproducibility
ProcedureĀ Overview
REAGENĀ Ā Sample and Folic acidĀ culture medium add to the 96-microwell which has the Lactobacillus RhamnoseĀ subsp. lactis ļ¼leichmanniiļ¼,if the sample has vitamin B9Ā the Lactobacillus RhamnoseĀ will grow,after culture 37ā,44-48h, read the OD by microplate reader 610 – 630 nmĀ (or540 – 550 nm),The sample of the turbidity compared with standard curve can get folic acid content.
Kit Contents, StorageĀ and Shelf Life
REAGENĀ Ā Folic acidĀ Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8ā .The shelf life is 6Ā months when the kit is properly stored.
Kit Contents | Amount | Storage |
Lactobacillus Rhamnose-Coated Plate | 1 x 96-well Plate (8 wells x 12 strips) | 2-8ā |
Sterile water | 3x30ml | 2-8ā |
Culture medium(containsB9Ā buffer) | 3 | 2-8ā |
Folic acid standards | 3 | 2-8ā |
Aluminum foil | 2 | 2-30ā |