Product Description

REAGEN  Vitamin B12 Test Kit is quantitative analysis of total Vitamin B12 in food ,feed and drug.

The unique features of the kit are:

1. rapid, and cost-effective extraction methods,the same as other Vitamin
2. The kit provide positive control and serial standards
3. High sensitivity (0.03μg/100g)
4. High recovery（90%-105%）
5. High reproducibility

Procedure Overview

REAGEN  Sample and Vitamin B12 culture medium add to the 96-microwell which has the Lactobacillus delbrueckii subsp. lactis （leichmannii）,if the sample has vitamin B12 the Lactobacillus delbrueckii will grow,after culture 37℃,44-48h, read the OD by microplate reader 610 – 630 nm (or540 – 550 nm),The sample of the turbidity compared with standard curve can get Vitamin B12 content.

Kit Contents, Storage and Shelf Life

REAGEN  Vitamin B12 Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8℃ .The shelf life is 6 months when the kit is properly stored.

Required Materials Not Provided With the Kit

1. Laminar flow bench
2. Microtiter plate reader：610 – 630 nm （540 – 550 nm）
3. Incubator：37 °C
4. Water bath 95℃
5. Centrifuge：8000g
6. Sterile Pipette 20-200ul,100-1000ul
7. Sterile centrifuge tubes (1.5-2ml  ,15 and 50 mL)
8. The 500 mL glass bottles and flasks (100 and 1000 mL), 100 mL beaker
9. Sterile syringe, 0.2 um sterile membrane
10. Sterile water ordeionized water
11. HCl：1mol/L and 0.1mol/L
12. NaOH：1mol/L and 0.1mol/L
13. NaCNor KCN：1%，100mg NaCN or KCN add 10ml sterile water
14. Amylase：z.B. Fluka 86250
15. Acetate buffer （pH4.5）,Add 0.66 g anhydrous sodium acetate to 100 mL beaker , add 50 mL sterile water or deionized water, stirring edge to dissolve, then add 0.69 mL of acetic acid (> 99%, such as j. t. Baker, 6052), the soluble liquid pH is 4.5, 0.1 mol/L HCl correction if necessary;Transfer buffer quantitative to 100 ml volumetric flask, with sterile water or deionized water constant volume.2-8 ℃can store 1 week.