Amantadine ELISA Test Kit


Amantadine ELISA Test Kit

Amantadine ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of amantadine in tissue、egg、feed.

Product Description

REAGEN  Amantadine ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of amantadine in tissue、egg、feed.

The unique features of the kit are:

  • High recovery(>75%), rapid (less than 60 minutes), and cost-effective extraction methods.
  • High sensitivity (0.5ng/g or ppb)
  • High reproducibility.
  • A quick ELISA assay (less than 2 hours regardless of number of samples).

Procedure Overview

REAGEN  Amantadine ELISA Test Kit is based on a competitive colorimetric ELISA assay. The plate wells are coated with anti-amantadine antibody. During analysis, sample is added along with the Amantadine-horseradish peroxidase (Amantadine-HRP) conjugate. If Amantadine residue is present in the sample, it will compete for the Amantadine antibody, thereby preventing the Amantadine-HRP from binding to the antibody attached to the well. The resulting color intensity, after addition of the HRP substrate (TMB), has an inverse relationship with the concentration of Amantadine residue in the sample.

Kit Contents, Storage and Shelf Life

REAGEN  Amantadine ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C . The shelf life is 12 months when the kit is properly stored.

Kit Contents Amount Storage
Amantadine Ab-Coated Plate 1 x 96-well plate (8 wells x 12 strips) 2-8℃
Amantadine Standards

Negative control

0.5 ng/mL

1.5 ng/mL



40.5 ng/mL

100 ng/mL(spiking, optional)
















 Antibody #1 5.5mL
 HRP-Conjugated Antibody #2 11mL 2-8℃
20× Wash Solution 40mL
Stop Buffer 6mL
TMB Substrate 12mL
2X Sample Dilution Buffer 50mL

If you are not planning to use the kit for over 1 month ,  Antibody #1 and HRP-Conjugated Antibody #2 at 20℃ or in a freezer is recommended.

Sensitivity (Detection Limit)

Sample Type Detection Limit (ng/g or ppb)
Tissue、Egg 1
Feed 5

Specificity (Cross-Reactivity)

Analytes Cross-Reactivity (%)
Amantadine 100






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