Product Description
REAGEN Beta-Agonist ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of clenbuterol, salbutamol, bitolterol, carbuterol and other related ß-agonists in feed, meat/liver/kidney, milk and urine.Beta-Agonist ELISA Test Kit enables international and government regulatory agencies, food manufacturers and processors, as well as quality assurance organizations, to detect Beta-Agonist in various sample types in response to customer concerns about food safety.
The unique features of the kit are:
- Rapid (10 – 30 minutes), and organic reagent-free extraction method for various samples withhigh recovery (75 – 105%).
- High sensitivity (0.05 ng/g or ppb) and low detection limit (0.1ng/g or ppb for meat/tissue, 0.05 ng/g or ppb for urine and 0 ng/g or ppb for feed).
- A quick ELISA assay (less than 2 hours regardless of number of samples.
- High reproducibility.
Procedure Overview
The method is based on a competitive colorimetric ELISA assay. The drug of interest has been coated in the plate wells. During the analysis, sample is added along with the primary antibody specific for the target drug. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the drug attached to the well. The secondary antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the drug coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.
Kit Contents, Storage and Shelf Life
REAGEN Beta-Agonist ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C *. The shelf life is 12 months when the kit is properly stored.
| Kit Contents | Amount | Storage |
| Beta-Agonist coated Microtiter Plate | 1 x 96-well plate (8 wells x 12 strips) | 2-8°C |
| Beta-Agonist Standards:
Negative control (white cap tube) 0.05 ng/mL (yellow cap tube) 0.15 ng/mL (orange cap tube) 0.5 ng/mL (pink cap tube) 1.5 ng/mL (purple cap tube) 4.5 ng/mL (blue cap tube) 10 ng/mL (spiking, optional, red cap tube) |
0.8 mL 0.8 mL 0.8 mL 0.8 mL 0.8 mL 0.8 mL 0.8 mL |
2-8°C |
| Beta-Agonist Antibody #1 | 15 mL | 2-8°C * |
| 100X HRP-Conjugated Antibody #2 | 250 mL | 2-8°C * |
| Antibody #2 Diluent ** | 20 mL | 2-8°C |
| 20X Sample Extraction Buffer ** | 10 mL | |
| 20X Wash Solution ** | 28 mL | |
| Stop Buffer ** | 15 mL | |
| TMB Substrate ** | 12 mL | |
| Sample Balance Buffer I ** | 10mL | |
| Sample Balance Buffer II(powder) ** | 62g | |
| 10X PBS ** | 25 mL | |
| Liver Extraction Buffer (Optional) | 10 mL | |
| Beta-Agonist Clean Up Buffer I (Optional) | 5 mL | |
| Beta-Agonist Clean Up Buffer II (Optional) | 5 mL | |
| Beta-Agonist Clean Up Buffer III (Optional) | 28 mL |
* If you are not planning to use the kit for over 3 months, store Beta-Agonist Antibody #1 and 100X HRP-Conjugated Antibody #2 at -20°C or in a freezer.
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