Product Description
REAGEN Diazepam ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of diazepam as well as other benzodiazepines in feed, meat (beef, chicken and pork), milk, serum and urine.
The unique features of the kit are:
- High recovery (>80%),rapid(10-30 minutes),and cost-effective extraction methods.
- High sensitivity(0.005ng/g or ppb).
- High reproducibility.
- A quick ELISA assay(less than 2 hours regardless of number of samples).
Procedure Overview
The method is based on a competitive colorimetric ELISA assay. The drug of interest has been coated in the plate wells. During the analysis, sample is added along with the primary antibody specific for the target drug. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the drug attached to the well. The secondary antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the drug coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.
Kit Contents, Storage and Shelf Life
REAGEN Diazepam ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C *. The shelf life is 12 months when the kit is properly stored.
| Kit Contents | Amount | Storage |
| Diazepam Coated Plate | 1 x 96-well Plate | 2-8°C |
| Diazepam Standards:
Negative control (white cap tube) 0.025ng/mL (yellow cap tube) 0.05 ng/mL (orange cap tube) 0.1 ng/mL (pink cap tube) 0.5 ng/mL (purple cap tube) 1.0 ng/mL (blue cap tube) 100 ng/mL (spiking, optional, red cap tube) |
1.0 mL 1.0 mL 1.0 mL 1.0 mL 1.0 mL 1.0 mL 1.0 mL
|
2-8°C * |
| Anti-Diazepam Antibody #1 | 12 mL | 2-8°C * |
| 100X HRP-Conjugated Antibody #2 | 250 mL | |
| Antibody #2 Diluent ** | 20 mL | |
| 20X Wash Solution ** | 30 mL | |
| TMB Substrate ** | 12 mL | |
| Stop Buffer ** | 14 mL | |
| 10X Sample Extraction Buffer ** | 25 mL |
* If you are not planning to use the kit for over 1 months, store Anti-Diazepam Antibody #1 and 100X HRP-Conjugated Antibody #2 at -20°C or in a freezer.
Sensitivity (Detection Limit)
| Sample Type | Detection Limit (g/g or ppb) |
| Feed | 0.25 |
| Meat/Liver/Kidney | 0.25 |
| Milk | 0.5 |
| Serum | 0.5 |
| Urine | 0.25 |
Specificity (Cross-Reactivity)
| Analytes | Cross-Reactivity (%) |
| Diazepam | 100 |
| Flunitrazepam | 100 |
| Lormetazepam | 100 |
| Triazolam | 25 |
| Alprazolam | 15 |
| Oxazepam | 1 |
| Chlordiazepoxide | <1 |
| Flurazepam | <1 |
| Bromazepam | <1 |
| Lorazepam | <1 |
| Clobazam | <1 |
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