Fumonisin Toxin ELISA Test

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Fumonisin Toxin ELISA Test

$999.00

Fumonisin ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Fumonisin in cereals, dried meat/fish, feed, seed.

Availability: 100 in stock (can be backordered)

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Product Description

REAGEN  Fumonisin ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Fumonisin in cereals, dried meat/fish, feed, seed.

The unique features of the kit are:

  • High recovery (80-120%),rapid(10-20minutes), cost-effective extraction methods.
  • High sensitivity (1 ng/g or ppb).
  • High reproducibility.
  • A quick ELISA assay (less than 1 hours regardless of number of samples).

Procedure Overview

The method is based on a competitive, one step colorimetric enzyme-linked immunosorbent assay (ELISA). The analyte-specific antibody has been coated to the plate wells. During analysis, sample is added along with the analyte-conjugated horseradish peroxidase (HRP). If the target analyte is present in the sample, it will compete for the antibody, thereby preventing the conjugated HRP from binding to the antibody coated to the plate well. The resulting color intensity, after addition of the TMB substrate, has an inverse relationship to the target analyte concentration in the sample. After loading prepared samples on the DS2 platform, the instrument handles all liquid handling, plate reading and data processing steps. The assay may also be run manually by the user.

Kit Contents, Storage and Shelf Life

REAGEN   Fumonisin ELISA Test Kit has the Fumonisinacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C *. The shelf life is 12 months when the kit is properly stored.

Kit Contents Amount Storage
Fumonisin  Antibody-Coated Plate 1 x 96-well Plate (8 wells x 12 strips) 2-8°C
Fumonisin Standards:

Negative control

1 ng/mL

3 ng/mL

9 ng/mL

27ng/mL

81 ng/mL

 

1.0 mL

1.0 mL

1.0 mL

1.0 mL

1.0 mL

1.0 mL

2-8°C

 

 

 

2-8°C

 

HRP-Conjugated 2# 5.5mL 2-8°C
Antibody 1# 5.5mL
20X Wash Solution ** 40 mL
Stop Buffer ** 6 mL
TMB Substrate ** 12 mL
10XSample Diluent 50mL

* If you are not planning to use the kit for over 3 months, store Fumonisin HRP-Conjugated  Antibody 1# at -20°C or in a freezer.

Sensitivity (Detection Limit)

Sample Type Detection Limit (ng/g or ppb)
Dried meat/Fish/Cereal/Feed/Seed 50

Specificity (Cross-Reactivity)

Analytes Cross-Reactivity (%)
Fumonisin B1 100.0
Fumonisin B2 94
Fumonisin B3 145
T-2 <0.1
DON <0.1

Brand

REAGEN

Model

RNM98009

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Product ImageFumonisin Toxin ELISA Test
$999.00

Availability: 100 in stock (can be backordered)