Product Description

REAGEN  Ochratoxin A ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Ochratoxin A in cereals, feed.

The unique features of the kit are:

1. High recovery (>80%),cost-effective extraction methods.
2. High sensitivity (1ng/g or ppb).
3. High reproducibility.
4. A quick ELISA assay (less than 1hours regardless of number of samples).

Procedure Overview

REAGEN  Ochratoxin A ELISA Test Kit is based on a competitive colorimetric ELISA assay. The toxin of interest has been coated in the plate wells. During the analysis, sample is added along with the primary antibody specific for the target toxin. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the toxin attached to the well. The second antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the toxin coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.

Kit Contents, Storage and Shelf Life

REAGEN  Ochratoxin A ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C *. The shelf life is 12 months when the kit is properly stored.

* If you are not planning to use the kit for over 3 months, store Ochratoxin A  Antibody #1 and HRP-Conjugated Antibody #2 at -20°C or in a freezer.

Sensitivity (Detection Limit)

Specificity (Cross-Reactivity)